Iwaoka Emiko, Oku Hisae, Takahashi Yumi, Ishiguro Kyoko
School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, Japan.
Biol Pharm Bull. 2009 Mar;32(3):509-12. doi: 10.1248/bpb.32.509.
Allergy-preventive activity was demonstrated for the MeOH extract (HM) of the petals of Hibiscus mutabilis L. 'versicolor' MAKINO in a continuing search for allergy-preventive substances from natural sources, using the in vivo assay method. This assay system uses monitoring of a decrease in the blood flow at the tail vein of mice subjected to hen egg-white lysozyme (HEL) sensitization. By bioassay-directed fractionation, a new flavonol triglycoside, quercetin 3-O-[beta-D-xylopyranosyl(1-->2)-alpha-L-rhamnopyranosyl(1-->6)]-beta-D-galactopyranoside (1: mutabiloside), was isolated, together with four known flavonols identified as quercetin 3-O-[beta-D-xylopyranosyl(1-->2)]-beta-D-galactopyranoside (2) and kaempferol 3-O-[beta-D-xylopyranosyl(1-->2)]-beta-D-galactopyranoside (3), quercetin (4) and hyperoside (5). The structure of the new flavonol 1 was elucidated by spectroscopic methods. Among these flavonol derivatives, compounds 1 and 2 showed significant allergy-preventive effects.
在持续从天然来源寻找抗过敏物质的过程中，利用体内测定法，对芙蓉（Hibiscus mutabilis L. 'versicolor' MAKINO）花瓣的甲醇提取物（HM）的抗过敏活性进行了验证。该测定系统通过监测对蛋清溶菌酶（HEL）致敏的小鼠尾静脉血流的减少情况来进行。通过生物测定导向的分级分离，分离出一种新的黄酮醇三糖苷，槲皮素3 - O - [β - D - 吡喃木糖基（1→2） - α - L - 吡喃鼠李糖基（1→6）] - β - D - 吡喃半乳糖苷（1：变色苷），以及四种已知的黄酮醇，分别鉴定为槲皮素3 - O - [β - D - 吡喃木糖基（1→2）] - β - D - 吡喃半乳糖苷（2）以及山奈酚3 - O - [β - D - 吡喃木糖基（1→2）] - β - D - 吡喃半乳糖苷（3）、槲皮素（4）和金丝桃苷（5）。通过光谱方法阐明了新黄酮醇1的结构。在这些黄酮醇衍生物中，化合物1和2显示出显著的抗过敏作用。
