Carrasco Adriano O T, Seki Meire C, de Sousa Ricardo L M, Raso Tânia F, Pinto Aramis A
Departamento de Medicina Veterinária, Universidade Estadual do Centro-Oeste-UNICENTRO, Guarapuava, PR, Brazil.
Trop Anim Health Prod. 2009 Oct;41(7):1325-33. doi: 10.1007/s11250-009-9318-7. Epub 2009 Mar 1.
The purposes of this study were to model a vaccination regimen for Newcastle disease virus (NDV) in pigeons, and to evaluate the susceptibility and behavior of vaccinated birds against a highly pathogenic NDV Brazilian strain. Antibody response was assessed by means of hemagglutination inhibition test (HI), and viral genome excretion by means of RT-PCR. Vaccinal strains (La Sota and Ulster) induced high antibody titers without any adverse effects, both in inoculated and in sentinel birds. A viral strain pathogenic for chickens did not produce clinical signs of the disease in experimentally infected pigeons. Only 4 out of 10 vaccinated pigeons shed NDV genome, and just for two days. Results confirmed the high infectivity of the vaccinal strains used, as all nonvaccinated pigeons showed antibody titers as high as those of vaccinated birds.
本研究的目的是为鸽子新城疫病毒（NDV）制定一种疫苗接种方案，并评估接种疫苗的鸽子对高致病性巴西新城疫病毒株的易感性和反应。通过血凝抑制试验（HI）评估抗体反应，通过逆转录聚合酶链反应（RT-PCR）评估病毒基因组排泄情况。疫苗株（La Sota和Ulster）在接种和哨兵鸽中均诱导出高抗体滴度，且无任何不良反应。一种对鸡致病的病毒株在实验感染的鸽子中未产生疾病临床症状。10只接种疫苗的鸽子中只有4只排出新城疫病毒基因组，且仅持续了两天。结果证实了所用疫苗株的高感染性，因为所有未接种疫苗的鸽子的抗体滴度与接种疫苗的鸽子一样高。
