Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel.
J Mol Neurosci. 2009 Sep;39(1-2):78-85. doi: 10.1007/s12031-009-9185-8. Epub 2009 Feb 28.
We have recently demonstrated that the rat ovary expresses LHbeta, FSHbeta, and the common alpha subunit mRNA. In the present report, we studied the regulation of LHbeta and of gonadotropin-releasing hormone (GnRH) mRNA expression in granulosa cells that were isolated from immature rats treated with either estrogen or pregnant mare serum gonadotropin (PMSG). In both cell types, GnRH agonist treatment resulted in a decrease in LHbeta mRNA expression. However, only in cells derived from PMSG-treated rats, GnRH treatment produced an increase in GnRH mRNA expression. A markedly increased GnRH mRNA expression was also obtained in granulosa cells derived from PMSG-primed rats in response to LH. In addition, FSH reduced the expression of LHbeta mRNA in granulosa cells from estrogen-primed rats. These results suggest that the expression of LHbeta in the ovary is regulated by locally produced GnRH and by FSH from either the ovary or the pituitary.
我们最近的研究表明，大鼠卵巢表达 LHβ、FSHβ 和共同的α亚基 mRNA。在本报告中，我们研究了从接受雌激素或孕马血清促性腺激素（PMSG）处理的未成熟大鼠中分离出的颗粒细胞中 LHβ 和促性腺激素释放激素（GnRH）mRNA 表达的调节。在这两种细胞类型中，GnRH 激动剂处理导致 LHβ mRNA 表达减少。然而，只有来自 PMSG 处理的大鼠的细胞中，GnRH 处理导致 GnRH mRNA 表达增加。在对 LH 反应的 PMSG 引发的大鼠颗粒细胞中，也获得了 GnRH mRNA 表达的显著增加。此外，FSH 减少了来自雌激素引发的大鼠颗粒细胞中 LHβ mRNA 的表达。这些结果表明，卵巢中 LHβ 的表达受局部产生的 GnRH 和来自卵巢或垂体的 FSH 调节。
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