Otlu Baris, Emre Sinan, Turkcuoglu Peykan, Doganay Selim, Durmaz Riza
Microbiology, Inonu University Medical Faculty, Turgut Ozal Medical Center, Research Hospital, Malatya - Turkey.
Eur J Ophthalmol. 2009 Mar-Apr;19(2):175-9. doi: 10.1177/112067210901900201.
Recent studies postulated the presence of a probable relationship between pterygium and neoplasia. This study aimed to investigate the role of two oncogenic viruses, human papillomavirus (HPV) and Epstein-Barr virus (EBV), in the development of conjunctival pterygia.
Polymerase chain reaction was used to identify the presence of HPV and EBV in 30 primary and 10 recurrent pterygia samples. Twenty conjunctival samples obtained from patients undergoing cataract surgeries were used as the control group. Patient groups had similar sex, race, and age distribution to eliminate bias. For exploration of HPV in groups, two different PCR methods (in-house PCR with two different primer sets and one real-time PCR method) were studied. The presence of EBV was shown by real-time PCR method.
HPV was identified in none of the pterygia and control group patients. However, EBV was detected in 3 out of 30 (10%) primary pterygia patients and in none of the recurrent pterygia and control patients.
Up to now, HPV has been blamed as the major viral pathogen in the etiopathogenesis of pterygium. The current results suggest that EBV may also be involved in the pathogenesis of pterygium, but further larger studies with larger cohorts are required to confirm this hypothesis.
近期研究推测翼状胬肉与肿瘤形成之间可能存在关联。本研究旨在调查两种致癌病毒，即人乳头瘤病毒（HPV）和爱泼斯坦-巴尔病毒（EBV）在结膜翼状胬肉发病过程中的作用。
采用聚合酶链反应来鉴定30例原发性和10例复发性翼状胬肉样本中HPV和EBV的存在情况。从接受白内障手术的患者中获取20份结膜样本作为对照组。患者组在性别、种族和年龄分布上相似，以消除偏差。为了在各组中检测HPV，研究了两种不同的PCR方法（使用两种不同引物组的内部PCR和一种实时PCR方法）。通过实时PCR方法显示EBV的存在情况。
在翼状胬肉组和对照组患者中均未检测到HPV。然而，在30例原发性翼状胬肉患者中有3例（10%）检测到EBV，而复发性翼状胬肉组和对照组患者中均未检测到。
到目前为止，HPV一直被认为是翼状胬肉发病机制中的主要病毒病原体。目前的结果表明，EBV也可能参与翼状胬肉的发病机制，但需要进一步开展更大规模的队列研究来证实这一假设。
