Arntzen Magnus Ø, Osland Christoffer Leif, Raa Christopher Rasch-Olsen, Kopperud Reidun, Døskeland Stein-Ove, Lewis Aurélia E, D'Santos Clive S
PROBE Proteomic Platform, Department of Biomedicine, University of Bergen, Bergen, Norway.
Proteomics. 2009 Mar;9(5):1400-6. doi: 10.1002/pmic.200800500.
Post-translationally modified peptides present in low concentrations are often not selected for CID, resulting in no sequence information for these peptides. We have developed a software POSTMan (POST-translational Modification analysis) allowing post-translationally modified peptides to be targeted for fragmentation. The software aligns LC-MS runs (MS(1) data) between individual runs or within a single run and isolates pairs of peptides which differ by a user defined mass difference (post-translationally modified peptides). The method was validated for acetylated peptides and allowed an assessment of even the basal protein phosphorylation of phenylalanine hydroxylase (PHA) in intact cells.
低浓度存在的翻译后修饰肽通常不会被选用于碰撞诱导解离（CID），导致这些肽没有序列信息。我们开发了一款软件POSTMan（翻译后修饰分析），可让翻译后修饰肽成为碎片化的目标。该软件能在单次运行内或不同单次运行之间比对液相色谱-质谱（LC-MS）运行结果（MS(1)数据），并分离出质量差异由用户定义的肽对（翻译后修饰肽）。该方法已针对乙酰化肽进行了验证，甚至能够评估完整细胞中苯丙氨酸羟化酶（PHA）的基础蛋白磷酸化情况。
