Wang Yuan, Gu Huixin, Lu Xin, Xu Guowang
Open Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Se Pu. 2008 Nov;26(6):649-57.
Hydrophilic interaction chromatography (HILIC) is popular in the analysis of hydrophilic solutes in recent years. As a unique liquid chromatographic (LC) separation mode, it has not only been successfully utilized for the separation of target compounds from complex matrixes, but also been hyphenated with other separation modes, such as reversed-phase LC (RPLC), ion-exchange chromatography, and various HILIC, to increase the resolution and peak capacity for "entire-component" analysis of complex samples. Here we briefly review the origin and concept of HILIC, its retention behavior, and the frequently used stationary phases. After discussing the selectivity orthogonality between HILIC and RPLC, several application areas of HILIC are introduced, including HILIC tandem mass spectrometry (HILIC-MS(n)), on-line HILIC pretreatment, comprehensive two-dimensional HILIC-MS(n) and on-line hyphenation of HILIC and RPLC. With abundant references, HILIC has been proved to be a powerful analytical tool for hydrophilic and polar complexes.
亲水作用色谱法（HILIC）近年来在亲水性溶质分析中颇受欢迎。作为一种独特的液相色谱（LC）分离模式，它不仅成功用于从复杂基质中分离目标化合物，还与其他分离模式联用，如反相液相色谱（RPLC）、离子交换色谱和各种亲水作用色谱，以提高复杂样品“全组分”分析的分辨率和峰容量。在此，我们简要回顾亲水作用色谱法的起源与概念、保留行为以及常用固定相。在讨论了亲水作用色谱法与反相液相色谱法之间的选择性正交性之后，介绍了亲水作用色谱法的几个应用领域，包括亲水作用色谱-串联质谱法（HILIC-MS(n)）、在线亲水作用色谱预处理、全二维亲水作用色谱-质谱法（HILIC-MS(n)）以及亲水作用色谱法与反相液相色谱法的在线联用。通过大量参考文献证明，亲水作用色谱法是分析亲水性和极性复合物的强大分析工具。
