Ranjane Poonam N, Gandhi Santosh V, Kadukar Sayali S, Ranher Sandeep S
Department of Pharmaceutical Analysis, A. I. S. S. M. S. College of Pharmacy, Pune-411001, India.
Se Pu. 2008 Nov;26(6):763-5. doi: 10.1016/s1872-2059(08)60033-4.
A simple, accurate and sensitive reversed-phase high performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of cefuroxime axetil and ornidazole in combined tablet dosage form has been developed. The method was performed with a HiQ-SiL C18 column (250 mm x 4.6 mm) and photodiode array (PDA) detector, using 0.01 mol/L potassium dihydrogen orthophosphate-methanol (56 : 44, v/v) as the mobile phase and tinidazole as the internal standard. Beer's law obeys in the concentration ranges of 5 - 25 microg/mL and 10 - 50 microg/mL for cefuroxime axetil and ornidazole, respectively. The method has been successfully validated statistically and applied for the analysis of the drugs in pharmaceutical formulation.
已开发出一种简单、准确且灵敏的反相高效液相色谱（RP-HPLC）法，用于同时测定复方片剂剂型中头孢呋辛酯和奥硝唑的含量。该方法采用HiQ-SiL C18柱（250 mm×4.6 mm）和光电二极管阵列（PDA）检测器，以0.01 mol/L磷酸二氢钾-甲醇（56∶44，v/v）为流动相，以替硝唑为内标。头孢呋辛酯和奥硝唑的浓度分别在5 - 25 μg/mL和10 - 50 μg/mL范围内符合比尔定律。该方法已成功通过统计学验证，并应用于药物制剂中药物的分析。
