Li Fang, Li Jiayang, Zhang Huayan, Guo Xingjie
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Se Pu. 2008 Nov;26(6):766-8.
A high performance liquid chromatographic (HPLC) method using amylase chiral stationary phase for the separation of bisoprolol enantiomers has been developed. Tris (3,5-dimethylphenyl carbamate) amylase chiral stationary phase was used in the normal-phase mode. The effects of the concentration of organic solvents (iso-propanol and ethanol), flow rate and column temperature on the enantiomer separation were studied systematically. The best separation was obtained with hexane-ethanol-diethyl amine (88 : 12 : 0.1, v/v/v) as mobile phase at 20 degrees C. The optimal flow rate was 0.6 mL/min. The detection wavelength was set at 270 nm. By using this method, the enantiomers of bisoprolol can be separated quickly and easily.
已开发出一种使用淀粉酶手性固定相分离比索洛尔对映体的高效液相色谱（HPLC）方法。三（3,5-二甲基苯基氨基甲酸酯）淀粉酶手性固定相用于正相模式。系统研究了有机溶剂（异丙醇和乙醇）浓度、流速和柱温对对映体分离的影响。在20℃下，以正己烷-乙醇-二乙胺（88:12:0.1，v/v/v）为流动相可获得最佳分离效果。最佳流速为0.6 mL/min。检测波长设定为270 nm。使用该方法，比索洛尔的对映体可快速、轻松地分离。
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