Chen Yuqing, Ma Zheng, An Fang, Guo Xingjie
Shenyang Pharmaceutical University, Shenyang 110016, China.
Se Pu. 2008 Sep;26(5):643-5.
A high performance liquid chromatographic method was established for the enantiomeric separation of liarozole. Baseline chiral separation of liarozole was achieved under normal-phase chromatographic mode by the Chiralpak AD-H chiral stationary phase. The influences of the concentration and kind of organic solvent, the proportion of acid and base, column temperature and flow rate on the enantiomeric separation were studied. The optimized chromatographic conditions were hexane-ethanol (containing 0.3% diethylamine and 0.1% glacial acetic acid) (80:20, v/v) as the mobile phase with a flow rate of 0.6 mL/min and detection at the wavelength of 254 nm. The column temperature was set at 20 degrees C. The resolution of 3.4 for liarozole was achieved under the above chromatographic conditions. The method is simple, rapid and with good reproducibility.
建立了一种用于分离利阿唑对映体的高效液相色谱法。采用Chiralpak AD-H手性固定相在正相色谱模式下实现了利阿唑的基线手性分离。研究了有机溶剂的浓度和种类、酸碱比例、柱温和流速对对映体分离的影响。优化的色谱条件为：以己烷-乙醇（含0.3%二乙胺和0.1%冰醋酸）（80:20，v/v）为流动相，流速为0.6 mL/min，检测波长为254 nm。柱温设定为20℃。在上述色谱条件下，利阿唑的分离度达到3.4。该方法简便、快速且重现性良好。
