Rung A, Miller D R, Scheffer S J
Plant Pest Diagnostics Branch, California Department of Food and Agriculture, 3294 Meadowview Rd., Sacramento, CA 95832-1448, USA.
J Econ Entomol. 2009 Feb;102(1):8-12. doi: 10.1603/029.102.0102.
The mealybug species Planococcus citri (Risso) and Planococcus minor (Maskell) (Hemiptera: Coccoidea: Pseudococcidae) have special significance to U.S. quarantine and U.S. agriculture. Commonly intercepted at U.S. ports-of-entry, they are difficult to identify based on morphological characters. This study presents a molecular method for distinguishing P. citri, P. minor, and a genetically distinct group that is morphologically identical to P. citri, from Hawaii. This method uses polymerase chain reaction (PCR) followed by restriction fragment polymorphism analysis (RFLP) using the restriction enzymes BspH1, BsmH1, and HpH1. The resulting band patterns can be visualized in a 2% agarose gel and are sufficient to differentiate between the three entities mentioned above. PCR-RFLP diagnostics can be used for all life stages and is cheaper and faster than DNA sequencing.
粉蚧类物种柑橘臀纹粉蚧（Planococcus citri (Risso)）和臀纹粉蚧（Planococcus minor (Maskell)）（半翅目：蚧总科：粉蚧科）对美国检疫和美国农业具有特殊意义。它们在美国入境口岸经常被截获，基于形态特征很难进行鉴定。本研究提出了一种分子方法，用于区分柑橘臀纹粉蚧、臀纹粉蚧以及来自夏威夷的与柑橘臀纹粉蚧形态相同但基因不同的群体。该方法采用聚合酶链反应（PCR），随后使用限制性内切酶BspH1、BsmH1和HpH1进行限制性片段多态性分析（RFLP）。所得条带模式可在2%琼脂糖凝胶中可视化，足以区分上述三个实体。PCR-RFLP诊断可用于所有生命阶段，且比DNA测序更便宜、更快。
