Chen Andrew C, He Haiqi, Temeyer Kevin B, Jones Shirley, Green Peter, Barker Stephen C
Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA-ARS, 2700 Fredericksburg Rd., Kerrville, TX 78028-9184, USA.
J Econ Entomol. 2009 Feb;102(1):373-80. doi: 10.1603/029.102.0148.
Mutations associated with pyrethroid resistance were found in Mexican strains of Rhipicephalus microplus (Canestrini). A mutation in the sodium channel gene was reported in strains highly resistant to permethrin and another mutation in an esterase gene in a strain that shows moderate resistance to the same pesticide. Methods based on the melting temperature difference of amplified allele-specific DNA fragments were developed that can detect these mutations rapidly in individual larvae. When these methods were applied to ticks from various strains of R. microplus from Australia, neither of these mutations could be demonstrated. Different resistance mechanisms have apparently developed independently between Australian and Mexican strains of R. microplus.
在微小扇头蜱（Canestrini）的墨西哥菌株中发现了与拟除虫菊酯抗性相关的突变。据报道，对氯菊酯高度抗性的菌株中钠通道基因发生了突变，而对同一杀虫剂表现出中度抗性的菌株中酯酶基因发生了另一种突变。开发了基于扩增等位基因特异性DNA片段熔解温度差异的方法，该方法可以在单个幼虫中快速检测到这些突变。当将这些方法应用于来自澳大利亚不同微小扇头蜱菌株的蜱虫时，未发现这些突变中的任何一种。显然，澳大利亚和墨西哥微小扇头蜱菌株之间独立发展出了不同的抗性机制。
