Koval'ova V A, Hut R T
Tsitol Genet. 2008 Nov-Dec;42(6):55-60.
A full length coding sequence corresponding to Scotch pine defensin 2 (PsDef2) was achieved by a PCR approach using P. sylvestris cDNA library as a template. It has the potential to encode a protein of 83 amino acids in length. The first 33 amino acids correspond to the N-terminal signal peptide. The mature protein contains domain gamma-thionin and possesses conserved residues which are common to all plant defensins.
以欧洲赤松cDNA文库为模板,通过PCR方法获得了与欧洲赤松防御素2(PsDef2)相对应的全长编码序列。它有可能编码一个长度为83个氨基酸的蛋白质。前33个氨基酸对应于N端信号肽。成熟蛋白包含γ-硫堇结构域,并具有所有植物防御素共有的保守残基。