Zhu Yu-chun, Tang Tie-long, Cui Shu, Zhou Bin, Wang Wei, Zeng Guo-jun, Lu Yi-ping
Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Nov;39(6):921-4.
To investigate the effects of ischemic postconditioning(IPo) on cellular apoptosis in acute renal ischemic-reperfusion injury (IRI) in rats.
Model of acute renal ischemic-reperfusion injury (IRI) was constructed using Wistar rats. Forty healthy male rats were divided into 4 groups randomly,including group C,group IRI, group IPo and group Ado. Rats of group C received right nephronectomy and separation of left renal vessels. Before reperfusion, group IPo was perfused for 10 s, following ischemic reperfusion for another 10 s with 6 cycles. Group Ado was infused with adenosine intravenously 10 min before ischemia. Cellular apoptosis was determined with TUNEL (TdT-mediated dUTP Nick End Labelling), flowcytometry and histopathological staining.
Compared with group C, the apoptotic index increased predominatly in group IRI (P<0.05), whereas, apoptosis index decreased obviousely in group IPo, Ado (P<0.05). In addition, the apoptosis index between group IPo and Ado was not statistically different.
Ischemic postconditioning could decrease cellular apoptosis induced by IRI in rat kidney.
探讨缺血后处理（IPo）对大鼠急性肾缺血再灌注损伤（IRI）细胞凋亡的影响。
采用Wistar大鼠构建急性肾缺血再灌注损伤（IRI）模型。40只健康雄性大鼠随机分为4组，即C组、IRI组、IPo组和Ado组。C组大鼠行右肾切除术并分离左肾血管。再灌注前，IPo组先灌注10秒，随后缺血再灌注10秒，共6个循环。Ado组在缺血前10分钟静脉注射腺苷。采用TUNEL（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法）、流式细胞术和组织病理学染色检测细胞凋亡。
与C组相比，IRI组凋亡指数显著升高（P<0.05），而IPo组和Ado组凋亡指数明显降低（P<0.05）。此外，IPo组和Ado组之间的凋亡指数无统计学差异。
缺血后处理可减少大鼠肾脏IRI诱导的细胞凋亡。
