Chu Chin-Ying, Lee Huei-Jane, Chu Chia-Yih, Yin Yu-Fang, Tseng Tsui-Hwa
Institute of Biochemistry and Biotechnology, Chung Shan Medical University, No. 110, Section 1, Chien-Kuo N. Road, Taichung, Taiwan.
Food Chem Toxicol. 2009 Jun;47(6):1265-71. doi: 10.1016/j.fct.2009.02.020. Epub 2009 Feb 28.
It has been reported that extracts of stem and leaf of Zanthoxylum ailanthoides (ZLE) possess antioxidative properties. However, the biological importance of the ZLE is not well known. In our preliminary study, it showed that ZLE prepared from 75% alcohol highly contains flavonoids (5.8%). By HPLC analysis, it shows that the ZLE consists of flavonoid glycosides including rutin and hyperoside. We investigate the effects of ZLE on the oxidation of low-density lipoprotein (LDL; d=1.019-1.063 g/mL) and the uptake of lipid in macrophage. Firstly, we explored the effect of ZLE on the oxidation of LDL by employing copper (II) sulfate (CuSO4) as an oxidative inducer. Oxidation was monitored by the formation of conjugated diene and thiobarbituric acid relative substances (TBARS), relative electrophoretic mobility (REM), and fragmentation of apolipoprotein B-100 (Apo B). Our data showed that ZLE reduced the oxidation properties of LDL induced by CuSO4. In addition, ZLE inhibited lipid accumulation in differentiated THP-1 cells treated with ox-LDL involving decreasing the expression of scavenger receptors such as scavenger receptor class AI (SR-AI) and CD36, which belongs to the class B scavenger receptor (SR-B). These results demonstrate the protective effect of ZLE on LDL oxidation and lipid accumulation in macrophage.
据报道，樗叶花椒（Zanthoxylum ailanthoides）的茎叶提取物（ZLE）具有抗氧化特性。然而，ZLE的生物学重要性尚不清楚。在我们的初步研究中，结果表明用75%乙醇制备的ZLE富含黄酮类化合物（5.8%）。通过高效液相色谱（HPLC）分析，结果显示ZLE由包括芦丁和金丝桃苷在内的黄酮苷组成。我们研究了ZLE对低密度脂蛋白（LDL；d = 1.019 - 1.063 g/mL）氧化以及巨噬细胞脂质摄取的影响。首先，我们以硫酸铜（CuSO4）作为氧化诱导剂，探究ZLE对LDL氧化的影响。通过共轭二烯和硫代巴比妥酸相关物质（TBARS）的形成、相对电泳迁移率（REM）以及载脂蛋白B - 100（Apo B）的片段化来监测氧化情况。我们的数据表明ZLE降低了CuSO4诱导的LDL的氧化特性。此外，ZLE抑制了用氧化型LDL（ox - LDL）处理的分化的THP - 1细胞中的脂质积累，这涉及降低清道夫受体如A类清道夫受体I（SR - AI）和CD36的表达，CD36属于B类清道夫受体（SR - B）。这些结果证明了ZLE对LDL氧化和巨噬细胞脂质积累具有保护作用。
