一种用于治疗研究的重组衰变加速因子复性的改进方法。

An improved method for refolding recombinant decay accelerating factor for therapeutic studies.

作者信息

Xu Jiang, Hu Yongliang, Gong Meiliang, Sun Chenjing, Xu Zhikai, Li Zhuyi, Wu Xingan

机构信息

Department of Neurology, Tangdu Hospital, The Fourth Military Medical University, Xi'An, China.

出版信息

Protein Expr Purif. 2009 Jul;66(1):102-6. doi: 10.1016/j.pep.2009.02.014. Epub 2009 Feb 28.

DOI:10.1016/j.pep.2009.02.014

PMID:19254764

Abstract

Decay accelerating factor (DAF) is a very potent complement regulatory protein which holds promise for clinical usage. Here we report on an improved procedure for refolding both rat and human DAF over-expressed in Escherichia coli. It was shown that 50-70% of the inclusion body could be refolded to soluble active protein. This method excludes the use of L-arginine, which is expensive, and can be used to prepare a large quantity of recombinant DAF for therapeutic studies.

摘要

衰变加速因子（DAF）是一种非常有效的补体调节蛋白，具有临床应用前景。在此，我们报告一种改进的方法，用于重折叠在大肠杆菌中过表达的大鼠和人DAF。结果表明，50%-70%的包涵体可以重折叠为可溶性活性蛋白。该方法不使用昂贵的L-精氨酸，可用于制备大量重组DAF用于治疗研究。