Xu Jiang, Hu Yongliang, Gong Meiliang, Sun Chenjing, Xu Zhikai, Li Zhuyi, Wu Xingan
Department of Neurology, Tangdu Hospital, The Fourth Military Medical University, Xi'An, China.
Protein Expr Purif. 2009 Jul;66(1):102-6. doi: 10.1016/j.pep.2009.02.014. Epub 2009 Feb 28.
Decay accelerating factor (DAF) is a very potent complement regulatory protein which holds promise for clinical usage. Here we report on an improved procedure for refolding both rat and human DAF over-expressed in Escherichia coli. It was shown that 50-70% of the inclusion body could be refolded to soluble active protein. This method excludes the use of L-arginine, which is expensive, and can be used to prepare a large quantity of recombinant DAF for therapeutic studies.
衰变加速因子（DAF）是一种非常有效的补体调节蛋白，具有临床应用前景。在此，我们报告一种改进的方法，用于重折叠在大肠杆菌中过表达的大鼠和人DAF。结果表明，50%-70%的包涵体可以重折叠为可溶性活性蛋白。该方法不使用昂贵的L-精氨酸，可用于制备大量重组DAF用于治疗研究。
