Wang Jun-Jie, Fu Xiu-Qing, Guo Yu-Guang, Yuan Lin, Gao Qian-Qian, Yu Hua-Li, Shi Heng-Liang, Wang Xing-Zhi, Xiong Wen-Cheng, Zhu Xiao-Juan
Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, PR China.
Cell Biol Int. 2009 May;33(5):578-85. doi: 10.1016/j.cellbi.2009.02.006. Epub 2009 Feb 28.
Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.
肌球蛋白X（Myo X）是一种具有尾部同源性4-带4.1/埃兹蛋白/根蛋白/膜突蛋白（MyTH4-FERM）尾部的非常规肌球蛋白，在各种哺乳动物组织中普遍表达。除了全长的Myo X（Myo X FL）外，大脑中还合成了一种无头形式。到目前为止，关于这种无运动功能的Myo X的功能知之甚少。在本研究中，我们在永生化促性腺激素释放激素（GnRH）神经元细胞NLT中研究了无头Myo X的作用。与野生型NLT细胞和表达绿色荧光蛋白（GFP）的NLT细胞相比，过表达无头Myo X的NLT细胞形成的粘着斑更少，铺展速度更慢。在化学迁移试验中，与对照NLT细胞相比，过表达无头Myo X的NLT细胞迁移的距离更短，迁移细胞更少。
