一项关于A-DNA晶体的初步中子晶体学研究。
A preliminary neutron crystallographic study of an A-DNA crystal.
作者信息
Leal Ricardo M F, Teixeira Susana C M, Blakeley Matthew P, Mitchell Edward P, Forsyth V Trevor
机构信息
ESRF, Grenoble, France.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Mar 1;65(Pt 3):232-5. doi: 10.1107/S1744309109002668. Epub 2009 Feb 12.
Abstract
The LADI-III diffractometer at the Institut Laue-Langevin has been used to carry out a preliminary neutron crystallographic study of the self-complementary DNA oligonucleotide d(AGGGGCCCCT)(2) in the A conformation. The results demonstrate the viability of a full neutron crystallographic analysis with the aim of providing enhanced information on the ion-water networks that are known to be important in stabilizing A-DNA. This is the first account of a single-crystal neutron diffraction study of A-DNA. The study was carried out with the smallest crystal used to date for a neutron crystallographic study of a biological macromolecule.
摘要
劳厄-朗之万研究所的LADI-III衍射仪已被用于对处于A构象的自互补DNA寡核苷酸d(AGGGGCCCCT)(2)进行初步的中子晶体学研究。结果表明，进行全面的中子晶体学分析以提供有关已知对稳定A-DNA很重要的离子-水网络的更多信息是可行的。这是关于A-DNA单晶中子衍射研究的首次报道。该研究使用了迄今为止用于生物大分子中子晶体学研究的最小晶体。
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