Hunkapiller T, Kaiser R J, Koop B F, Hood L
NSF Science and Technology Center for Molecular Biotechnology, California Institute of Technology, Pasadena 91125.
Science. 1991 Oct 4;254(5028):59-67. doi: 10.1126/science.1925562.
DNA sequence analysis is a multistage process that includes the preparation of DNA, its fragmentation and base analysis, and the interpretation of the resulting sequence information. New technological advances have led to the automation of certain steps in this process and have raised the possibility of large-scale DNA sequencing efforts in the near future [for example, 1 million base pairs (Mb) per year]. New sequencing methodologies, fully automated instrumentation, and improvements in sequencing-related computational resources may render genome-size sequencing projects (100 Mb or larger) feasible during the next 5 to 10 years.
DNA序列分析是一个多阶段过程，包括DNA的制备、片段化和碱基分析，以及对所得序列信息的解读。新技术进展已使该过程中的某些步骤实现自动化，并增加了在不久的将来进行大规模DNA测序工作的可能性[例如，每年100万个碱基对（Mb）]。新的测序方法、全自动仪器以及测序相关计算资源的改进，可能会使基因组大小的测序项目（100 Mb或更大）在未来5至10年内变得可行。
