Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32610-0126, USA.
Vet Res Commun. 2009 Oct;33(7):645-57. doi: 10.1007/s11259-009-9213-8. Epub 2009 Mar 3.
The present in vitro study was designed to evaluate whether canine bone marrow stromal cells (BMSCs) promote neurite outgrowth from dorsal root ganglion (DRG) neurons. Bone marrow aspirates were collected from iliac crests of three young adult dogs. DRG neurons were cultured on BMSCs, fibroblasts, or laminin substrates. DRG neurons were also cultured in BMSC- or fibroblast-conditioned media. DRG neurons grown on BMSCs extended longer neurites and developed a much more elaborate conformation of branching neurites compared to those on fibroblasts or laminin. Quantitative analysis revealed that these effects were associated with the emergence of increased numbers of primary and branching neurites. The effect appears to be dependent upon cell-cell interactions rather than by elaboration of diffusible molecules. With more extensive investigations into the basic biology of canine BMSCs, their ability for promoting neurite outgrowth may be translated into a novel therapeutic strategy for dogs with a variety of neurological disorders.
本体外研究旨在评估犬骨髓基质细胞（BMSCs）是否促进背根神经节（DRG）神经元的轴突生长。从三只年轻成年犬的髂嵴采集骨髓抽吸物。将 DRG 神经元培养在 BMSCs、成纤维细胞或层粘连蛋白底物上。还将 DRG 神经元培养在 BMSC 或成纤维细胞条件培养基中。与成纤维细胞或层粘连蛋白相比，在 BMSCs 上生长的 DRG 神经元延伸出更长的轴突，并发育出更加精细的分支轴突构象。定量分析表明，这些效应与出现更多的初级和分支轴突有关。该作用似乎依赖于细胞-细胞相互作用，而不是通过可扩散分子的发挥作用。随着对犬 BMSCs 基础生物学的更广泛研究，它们促进轴突生长的能力可能转化为治疗各种神经疾病犬的新治疗策略。
