He Ruo, Jiang Chen-jing, Wang Jing, Gao Qing-jun, Shen Dong-sheng
Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China.
Huan Jing Ke Xue. 2008 Dec;29(12):3574-9.
As compared with the ordinary landfill cover material, clay soil, the effect of methane stress on oxidation rate and microbial community structure was investigated in waste soil (material from biologically treated municipal solid waste). The results showed that the moisture content of the clay soil was low, due to the low water retaining capacity. As environmental temperature and rainfall changed, the clay soil caked and inhibited methanotrophs growth. However, with a high organic matter, water-holding capacity and porosity, the waste soil provided a favor condition for methanotrophs growth and propagation. After exposure to methane flow for 120 days, methane oxidation potential in the middle and bottom layers of the waste soil column increased to 11.25-13.48 micromol/(g x h), which was 10.4-24.5 times higher than that in clay soil column. The topsoils were both found to be dried and inhibit methane oxidation. Methane oxidation (removal) efficiency by the waste soil column reached 48.3% at the end of the experiment, which was 5-6 times higher than that by the clay soil column. The amounts of the phospholipid fatty acid (PLFA) biomarks 16:1 omega 8c and 18:1 omega 8c for Type I and II methanotrophs, respectively, showed that a strong linear relationship was observed between methane oxidation potential and PLFA 18:1 omega 8c content in soil samples.
与普通填埋覆盖材料黏土相比，研究了甲烷胁迫对废弃土壤（来自生物处理后的城市固体废弃物的材料）氧化速率和微生物群落结构的影响。结果表明，黏土的含水量较低，这是由于其保水能力较差。随着环境温度和降雨量的变化，黏土会结块并抑制甲烷氧化菌的生长。然而，废弃土壤含有较高的有机质、持水能力和孔隙度，为甲烷氧化菌的生长和繁殖提供了有利条件。在甲烷气流作用120天后，废弃土壤柱中层和底层的甲烷氧化潜力增加到11.25 - 13.48微摩尔/（克·小时），这比黏土土壤柱中的高出10.4 - 24.5倍。表层土壤均被发现干燥且抑制甲烷氧化。在实验结束时，废弃土壤柱的甲烷氧化（去除）效率达到48.3%，这比黏土土壤柱高出5 - 6倍。分别代表Ⅰ型和Ⅱ型甲烷氧化菌的磷脂脂肪酸（PLFA）生物标志物16:1 ω8c和18:1 ω8c的含量表明，土壤样品中的甲烷氧化潜力与PLFA 18:1 ω8c含量之间存在很强的线性关系。
