Department of Environmental Engineering, Zhejiang University, Hangzhou, China.
J Appl Microbiol. 2013 Sep;115(3):756-65. doi: 10.1111/jam.12263. Epub 2013 Jun 19.
Aerobic CH4 oxidation is an important process controlling CH4 release from landfills to the atmosphere. The aim of this study was to investigate the link between CH4 oxidation activity and methanotrophs abundance and diversity in landfill cover soils of different age.
Among the three investigated sites, the highest CH4 emission occurred at the active landfill area with the range of 1371-2242 mg m(-2) day. The CH4 oxidation activities of landfill cover soils were 1·07-1·21 μmol g(-1) h(-1) in the landfill area of 7-16 years, which was 7-17 times higher than those in the active landfill area. The relative abundance of methanotrophs assessed by quantification of pmoA gene was about 1·7 × 10(6) -2·4 × 10(7) copies g(-1) in the landfill cover soils. The CH4 oxidation activity was positively correlated with pmoA copy number in the landfill cover soil of each site, respectively. Type II methanotrophs (Methylocystis) and type I methanotrophs including Methylosoma, Methylocaldum and Methylococcus were all present in the landfill cover soils. Compared to type I methanotroph, type II methanotroph, Methylocystis, was more abundant in the acidic landfill cover soils.
Oxidation activity and community structure of methanotrophs varied with depth and age of landfill cover soils.
These findings provide new fundamental information regarding the activity and diversity of methanotrophs in landfill cover soils of different age that may aid predicting and modelling CH4 flux from landfills.
好氧 CH4 氧化是控制垃圾填埋场向大气中 CH4 释放的重要过程。本研究旨在探讨不同年龄填埋场覆盖土壤中 CH4 氧化活性与甲烷营养菌丰度和多样性之间的关系。
在所研究的三个地点中，活性垃圾填埋区的 CH4 排放量最高，范围为 1371-2242 mg m(-2) 天。填埋场覆盖土壤的 CH4 氧化活性在 7-16 年的填埋场区域为 1.07-1.21 μmol g(-1) h(-1)，比活性填埋场区域高 7-17 倍。通过 pmoA 基因定量评估，甲烷营养菌的相对丰度在填埋场覆盖土壤中约为 1.7×10(6) -2.4×10(7) 拷贝 g(-1)。在每个地点的填埋场覆盖土壤中，CH4 氧化活性与 pmoA 拷贝数呈正相关。在填埋场覆盖土壤中均存在 II 型甲烷营养菌（Methylocystis）和包括 Methylosoma、Methylocaldum 和 Methylococcus 在内的 I 型甲烷营养菌。与 I 型甲烷营养菌相比，II 型甲烷营养菌，即 Methylocystis，在酸性填埋场覆盖土壤中更为丰富。
氧化活性和甲烷营养菌的群落结构随填埋场覆盖土壤的深度和年龄而变化。
这些发现为不同年龄填埋场覆盖土壤中甲烷营养菌的活性和多样性提供了新的基础信息，这可能有助于预测和模拟垃圾填埋场的 CH4 通量。
