de Albuquerque Sérgio, Lopes Ruberval Armando, Sala Miguel Angel, Abrahão Ana Amélia Carraro, Rosa Domingues Ribeiro
Departamento de Análisis Clinicos y Bromatológicos, Facultad de Ciencias Farmacéuticas de Ribeirão Preto, USP, Brasil.
Rev Biol Trop. 2008 Jun;56(2):459-71.
The aim of this work was to analyze histologically and histometrically the sublingual gland of mice infected with the RAL strain of T. cruzi, according to the sex. Swiss mice (Mus musculus) were inoculated with 2 x 10(4) blood trypomastigotes of the RAL strain of T. cruzi. In the peak of the parasitemia (12th day) the mice were sacrificed, and the sublingual glands were fixed in ALFAC. HE-stained histological sections were evaluated histometrically. The parasitemia was higher in females. Histopatologically, acini of the infected animals were smaller, with scanty production of secretion, and smaller striated ducts. The nuclei of the demilunes were smaller and showed amastigote nests in the cytoplasm. Karyometrically, nuclei of the acini, demilunes and striated ducts were smaller in the infected mice. Stereologically, it was observed that relative volumes of acini and ducts were smaller and, inversely, relative volumen were greater for the conjunctive tissue in the infected males. The surface densities of acini and ducts were bigger and the diameter and thickness of the wall were smaller in this group. On the other hand, relative volume of acini was smaller and those of the ducts and conjunctive tissue were bigger in the infected females. The diameter and thickness of the wall of acini were smaller, and those of the striated ducts were bigger in this group. The RAL strain of T. cruzi caused general atrophy in the sublingual gland, with numerous nests of parasites in the glandular parenchyma.
本研究旨在根据性别，对感染克氏锥虫RAL株的小鼠舌下腺进行组织学和组织计量学分析。将瑞士小鼠（小家鼠）接种2×10⁴个克氏锥虫RAL株的血液型锥鞭毛体。在寄生虫血症高峰期（第12天）处死小鼠，将舌下腺固定于ALFAC中。对苏木精-伊红染色的组织切片进行组织计量学评估。雌性小鼠的寄生虫血症更高。组织病理学上，感染动物的腺泡较小，分泌物产生稀少，纹状管也较小。半月细胞的细胞核较小，细胞质中可见无鞭毛体巢。核测量学结果显示，感染小鼠的腺泡、半月细胞和纹状管的细胞核较小。体视学观察发现，感染雄性小鼠的腺泡和导管的相对体积较小，相反，结缔组织的相对体积较大。该组中腺泡和导管的表面密度较大，壁的直径和厚度较小。另一方面，感染雌性小鼠的腺泡相对体积较小，导管和结缔组织的相对体积较大。该组中腺泡壁的直径和厚度较小，纹状管的直径和厚度较大。克氏锥虫RAL株导致舌下腺普遍萎缩，腺实质中有大量寄生虫巢。
