Borraz-Argüello María del Tránsito, Santos-López Gerardo, Vallejo-Ruiz Verónica, Herrera-Camacho Irma, Reyes-Leyva Julio
Laboratorio de Biología Molecular y Virologia, Centro de Investigación Biomédica de Oriente, IMSS, HGZ No. 5, Km. 4.5 Carretera Federal Atlixco-Metepec, 74630 Metepec, Puebla, México.
Rev Biol Trop. 2008 Jun;56(2):487-99.
Biological characterization of three natural isolates of the porcine rubulavirus (Mexico). Porcine rubulavirus (PoRV) produces a neurological and reproductive syndrome in pigs called the blue-eye disease, known only from Mexico. Several isolates were grouped by the main symptoms presented during outbreaks: a) neurotropic in piglets, (b) broadly neurotropic in piglets and gonadotropic in adults, and (c) gonadotropic in adults. We studied some biological properties of three strains, which fall in one of each virus group: La Piedad Michoacán (LPM) and Producci6n Animal Cerdos 1 (PAC1) and 3 (PAC3), respectively. The analyzed viral properties are mainly related with the trans-membrane hemagglutinin-neuraminidase (HN) and fusion (F) proteins, such as cytopathic effect, hemolysis, hemagglutinating (HA) and neuraminidase (NA) activities. In the infection assays PAC1 strain presented the highest fusogenicity level; however, the most cytolytic strain was PAC3. In addition, HA and NA activities and viral genome of PAC3 strain was detected in supernatants during cell infection earlier than in the other two strains, which shows that PAC3 virions release from the host cell earlier than LPM and PAC1. Experimental determination in purified viruses shows that PAC3 presented a higher HA and NA activities; however, PAC1 shows other interesting properties, such as a high thermostability of HN and differences about substrate profile respect to LPM and PAC3. Our data suggest that NA activity is associated with the virulence of RVP.
猪腮腺炎病毒（墨西哥）三种自然分离株的生物学特性。猪腮腺炎病毒（PoRV）在猪中引发一种神经和生殖系统综合征，称为蓝眼病，仅在墨西哥有此病例。根据疫情爆发期间出现的主要症状，将几种分离株进行了分组：a）对仔猪具有嗜神经性，（b）对仔猪具有广泛嗜神经性且对成年猪具有促性腺性，以及（c）对成年猪具有促性腺性。我们研究了三种毒株的一些生物学特性，它们分别属于每个病毒组中的一种：分别是米却肯州拉彼达（LPM）、动物生产猪1号（PAC1）和3号（PAC3）。所分析的病毒特性主要与跨膜血凝素神经氨酸酶（HN）和融合（F）蛋白有关，如细胞病变效应、溶血、血凝（HA）和神经氨酸酶（NA）活性。在感染试验中，PAC1毒株表现出最高的融合活性水平；然而，细胞溶解性最强的毒株是PAC3。此外，在细胞感染期间，PAC3毒株的上清液中比其他两种毒株更早检测到HA和NA活性以及病毒基因组，这表明PAC3病毒粒子比LPM和PAC1更早从宿主细胞释放。对纯化病毒的实验测定表明，PAC3表现出更高的HA和NA活性；然而，PAC1显示出其他有趣的特性，如HN的高热稳定性以及与LPM和PAC3相比底物谱的差异。我们的数据表明，NA活性与RVP的毒力相关。
