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均相蛋白质溶液中的粘弹性。

Viscoelasticity in homogeneous protein solutions.

作者信息

Pan Weichun, Filobelo Luis, Pham Ngoc D Q, Galkin Oleg, Uzunova Veselina V, Vekilov Peter G

机构信息

Department of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas 77204-4004, USA.

出版信息

Phys Rev Lett. 2009 Feb 6;102(5):058101. doi: 10.1103/PhysRevLett.102.058101. Epub 2009 Feb 2.

Abstract

We probe the transport properties in protein solutions stable with respect to any, solid or liquid, phase separation as a step in the understanding of transport in the cytosol of live cells. We determine the mean-squared displacement of probe particles in the time range 10;{-3}-10 s in solutions of a model protein. The tested solutions exhibit significant elasticity at high frequencies, while at low frequencies, they are purely viscous. We attribute this viscoelasticity to a dense network of weakly-bound chains of protein molecules with characteristic lifetime of 10-100 ms. The found intrinsic viscoelasticity of protein solutions should be considered in biochemical kinetics models.

摘要

我们研究了在任何固液相分离方面都稳定的蛋白质溶液中的输运性质,以此作为理解活细胞胞质溶胶中输运的一个步骤。我们测定了模型蛋白质溶液中探针粒子在10⁻³ - 10秒时间范围内的均方位移。测试溶液在高频下表现出显著的弹性,而在低频下则纯粹是粘性的。我们将这种粘弹性归因于蛋白质分子弱结合链的密集网络,其特征寿命为10 - 100毫秒。在生化动力学模型中应考虑所发现的蛋白质溶液的固有粘弹性。

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