Sengupta Sonali, Parihar Rashmi, Ganesh Subramaniam
Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208 016, India.
Biochem Biophys Res Commun. 2009 Apr 24;382(1):102-7. doi: 10.1016/j.bbrc.2009.02.137. Epub 2009 Mar 1.
The heat shock response in human cells is associated with the transcription of satellite III repeats (SatIII) located in the 9q12 locus. Upon induction, the SatIII transcripts remain associated with the locus and recruit several transcription and splicing factors to form the nuclear stress bodies (nSBs). The nSBs are thought to modulate epigenetic changes during the heat shock response. We demonstrate here that the nSBs are induced by a variety of stressors and show stress-specific patterns of induction. While the transcription factor HSF1 is required for the induction of SatIII locus by the stressors tested, its specific role in the transcriptional process appears to be stress dependent. Our results suggest the existence of multiple transcriptional loci for the SatIII transcripts and that their activation might depend upon the type of stressors. Thus, induction of SatIII transcripts appears to be a generic response to a variety of stress conditions.
人类细胞中的热休克反应与位于9q12位点的卫星III重复序列（SatIII）的转录相关。诱导后，SatIII转录本仍与该位点相关联，并招募多种转录和剪接因子以形成核应激小体（nSBs）。核应激小体被认为在热休克反应过程中调节表观遗传变化。我们在此证明，核应激小体由多种应激源诱导，并呈现应激特异性的诱导模式。虽然转录因子HSF1是所测试的应激源诱导SatIII位点所必需的，但其在转录过程中的具体作用似乎取决于应激类型。我们的结果表明存在多个SatIII转录本的转录位点，并且它们的激活可能取决于应激源的类型。因此，SatIII转录本的诱导似乎是对多种应激条件的一种普遍反应。
