Moussalli Adnan, Herbert David G, Stuart-Fox Devi
School of Biological and Conservation Sciences, University of KwaZulu-Natal, Pietermaritzburg 3206, South Africa.
Mol Phylogenet Evol. 2009 Jul;52(1):167-82. doi: 10.1016/j.ympev.2009.02.018. Epub 2009 Mar 1.
The genus Natalina Pilsbry, 1893 is a southern African endemic belonging to the Gondwanan family of carnivorous snails, Rhytididae. We present a well-resolved molecular phylogeny of the genus based on the mitochondrial 16S and COI genes and the nuclear ITS2 gene, and assess this in light of Watson's [Watson, H., 1934. Natalina and other South African snails. Proc. Malacol. Soc. Lond. 21, 150-193] supra-specific classification via a re-examination of 23 morphological characters including features of the shell, radula, external anatomy and distal reproductive tract. Ancestral reconstruction and character mapping based on the MK(1) model reveals broad concordance between morphology and the molecular phylogeny at the supra-specific level. Given this concordance and exceptionally deep divergences in the molecular data, we recommend the elevation of the subgenera Natalina s.s., Afrorhytida, and Capitina to generic status. At the species level, we identify several species complexes for which additional fine scale morphological and molecular appraisal is needed to qualify on the one hand incipient speciation with notable differentiation in shell form and body pigmentation, and on the other, phylogenetically deep yet morphologically cryptic diversity.
纳塔利纳属（Natalina Pilsbry，1893）是一种非洲南部特有的物种，属于冈瓦纳肉食性蜗牛科——皱纹螺科（Rhytididae）。我们基于线粒体16S和COI基因以及核ITS2基因，构建了该属解析度良好的分子系统发育树，并根据沃森[沃森，H.，1934年。《纳塔利纳和其他南非蜗牛》。《伦敦软体动物学会会刊》21，150 - 193]的超种分类，重新审视了23个形态特征，包括贝壳、齿舌、外部解剖结构和远端生殖道的特征。基于MK(1)模型的祖先重建和特征映射显示，在超种水平上，形态学与分子系统发育之间存在广泛的一致性。鉴于这种一致性以及分子数据中异常深的分歧，我们建议将纳塔利纳属（狭义）、非洲皱纹螺亚属（Afrorhytida）和 Capitina亚属提升为属级地位。在物种水平上,我们识别出几个物种复合体，一方面，对于贝壳形态和身体色素沉着有显著差异的初始物种形成，另一方面，对于系统发育上分歧较大但形态上难以区分的多样性，都需要进行额外的精细尺度形态学和分子评估。
