Sawai Chihiro, Takano Tomoyuki, Takeuchi Yoshihiro
Department of Pediatrics, Shiga University of Medical Science, Seta-Tsukinowa, Otsu, Japan.
J Child Neurol. 2009 Mar;24(3):275-86. doi: 10.1177/0883073808323028.
To examine the role of subventricular zone in cortical dysplasia, an experimental model was established using a neuronal tracing study. Ibotenate is a glutamate receptor agonist, and its intracerebral injection produces excitotoxic lesions mimicking microgyria and heterotopia. A total of 72 newborn hamsters were subjected. Biotinylated dextran amine was injected into the ganglionic eminence for the neuronal tracer. The cortical lesions and apoptotic cells were analyzed. In the microgyric cortex, terminal deoxynucleotidyl transferase-mediated 2' -deoxyuridine 5'-triphosphate-biotin end labeling-positive cells were increased and accumulated in the cortical folding. Biotinylated dextran amine-positive cells and fibers were derived from the dorsolateral subventricular zone and directed toward the cortical folding. In the heterotopic cortex, terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate- biotin end labeling-positive cells were distributed around the heterotopia, but number of cells was not remarkable. Our findings suggest that the subventricular zone plays an important role in the formation of cortical dysplasia, especially in the microgyria, after excitatory neuronal injury.
为研究脑室下区在皮质发育异常中的作用，采用神经示踪研究建立了一个实验模型。异搏定是一种谷氨酸受体激动剂，脑内注射异搏定可产生类似微小脑回和异位的兴奋性毒性损伤。共纳入72只新生仓鼠。将生物素化葡聚糖胺注入神经节隆起作为神经示踪剂。对皮质损伤和凋亡细胞进行分析。在微小脑回皮质中，末端脱氧核苷酸转移酶介导的生物素化2'-脱氧尿苷5'-三磷酸末端标记阳性细胞增多，并聚集在皮质折叠处。生物素化葡聚糖胺阳性细胞和纤维源自背外侧脑室下区，并指向皮质折叠处。在异位皮质中，末端脱氧核苷酸转移酶介导的生物素化2'-脱氧尿苷5'-三磷酸末端标记阳性细胞分布在异位灶周围，但细胞数量不显著。我们的研究结果表明，在兴奋性神经元损伤后，脑室下区在皮质发育异常的形成中起重要作用，尤其是在微小脑回的形成中。
