Mojtahedi H, Boydston R A, Crosslin J M, Brown C R, Riga E, Anderson T L, Spellman D, Quick R A
USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350 Washington State University, IAREC, 24106 N. Bunn Rd., Prosser, WA 99350. E. Riga is supported by the College of Agricultural, Human, and Natural Resource Sciences Agricultural Research Centre, PPNS No. 0452, Department of Plant Pathology and IAREC WSU-Prosser, Project No.WNP00542, Washington State University, Pullman, WA 99164-6430, USA.
J Nematol. 2007 Dec;39(4):313-6.
A method to establish two experimental corky ringspot disease (CRS) plots that had no prior CRS history is described. CRS is a serious disease of potato in the Pacific Northwest caused by tobacco rattle virus (TRV) and transmitted primarily by Paratrichodorus allius. 'Samsun NN' tobacco seedlings were inoculated with viruliferous P. allius in the greenhouse before they were transplanted into the field soil at the rate of 3,000 plus seedlings/ha. Care was taken to keep soil around plants in the greenhouse and transplants in the field moist to avoid vector mortality. The vector population in the soil of one of the fields was monitored by extraction, examination under microscope and bioassay on tobacco seedlings to ascertain that they were virus carriers. Presence of virus in tobacco bioassay plants was determined by visual symptoms on tobacco leaves and by testing leaves and roots using ELISA. Although TRV transmission was rapid, there was loss of infectivity in the first winter which necessitated a re-inoculation. After two years of planting infected tobacco seedlings, 100% of soil samples collected from this field contained viruliferous P. allius. In the second field, all five commercial potato cultivars, known to be susceptible, expressed symptoms of CRS disease indicating that the procedure was successful.
本文描述了一种建立两个此前无软木栓环斑病（CRS）病史的实验性CRS地块的方法。CRS是太平洋西北地区一种严重的马铃薯病害，由烟草脆裂病毒（TRV）引起，主要由葱短体线虫传播。在温室中，将带毒的葱短体线虫接种到“萨姆松NN”烟草幼苗上，然后以每公顷3000多株幼苗的速度将其移植到田间土壤中。注意保持温室中植株周围的土壤以及田间移植苗的湿润，以避免传毒介体死亡。通过提取、显微镜检查和在烟草幼苗上进行生物测定来监测其中一块田地土壤中的传毒介体种群，以确定它们是否为病毒携带者。通过烟草叶片上的可见症状以及使用酶联免疫吸附测定（ELISA）检测叶片和根部，来确定烟草生物测定植株中是否存在病毒。尽管TRV传播迅速，但在第一个冬季感染力有所丧失，因此需要重新接种。在种植感染烟草幼苗两年后，从该田地采集的100%土壤样本中都含有带毒的葱短体线虫。在另一块田地中，所有五个已知易感的商业马铃薯品种都表现出CRS病症状，表明该方法是成功的。
