Sun Xue-hui, Guo Tao, He Jin, Zhao Ming-hong, Nie Shu-fang
Department of Pharmacy, General Hospital of Shenyang Military Region, Shenyang 110016, China.
Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(23):2772-5.
To investigate the tissue distribution of the diallyl disulfide (DADS) and diallyl trisulfide (DATS) in solid lipid nanoparticles loaded garlic oil (GO-SLN) in rats.
The gas chromatography-electron capture detection (GC-ECD) method was established to determined the DADS and DATS simultaneously in the biological samples of rats after administration of 0.5 mL garlic oil injection or GO-SLN (containing about 10 mg garlic oil) via jugular vein cannula. The conditions for gas chromatographic separation were as follows. The oven temperature was set at 110 degrees C and maintained for 15 min. Temperatures at the injection port and detector were 180 degrees C and 300 degrees C, respectively. Ultra-pure nitrogen (purity > 99.999%, Shenyang Kerui Special Gases Co. Ltd., China) was used as a carrier gas and made-up gas at flow-rates of 1 mL x min(-1) and 60 mL x min(-1), respectively. All injections were carried out in the split injection mode with a split ratio of 1:10.
The GC-ECD method was fit for determing the concentration of DADS and DATS in garlic oil. The distribution character of GO-SLN in rats had changed to some extent and the concentration of GO-SLN in tissues was higher than that of GO-Injection.
The SLN can elevate the passive targeting of drugs and lengthen their action time in tissues.
研究大蒜油固体脂质纳米粒（GO-SLN）中大蒜素二烯丙基二硫化物（DADS）和二烯丙基三硫化物（DATS）在大鼠体内的组织分布。
建立气相色谱-电子捕获检测（GC-ECD）法，用于同时测定大鼠经颈静脉插管注射0.5 mL大蒜油注射液或GO-SLN（含约10 mg大蒜油）后生物样品中的DADS和DATS。气相色谱分离条件如下：柱温箱温度设定为110℃，保持15分钟。进样口和检测器温度分别为180℃和300℃。超纯氮气（纯度>99.999%，中国沈阳科瑞特种气体有限公司）分别以1 mL·min⁻¹和60 mL·min⁻¹的流速作为载气和补充气。所有进样均采用分流进样模式，分流比为1:10。
GC-ECD法适用于测定大蒜油中DADS和DATS的浓度。GO-SLN在大鼠体内的分布特征有一定变化，其在组织中的浓度高于大蒜油注射液。
固体脂质纳米粒可提高药物的被动靶向性并延长其在组织中的作用时间。
