Liu Man, Yuan Li, Liu Nai-You, Shi Dong-Qiao, Liu Jie, Yang Wei-Cai
Key Laboratory of Molecular and Developmental Biology, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
J Integr Plant Biol. 2009 Mar;51(3):261-71. doi: 10.1111/j.1744-7909.2008.00783.x.
RNA biogenesis is essential and vital for accurate expression of genes. It is obvious that cells cannot continue normal metabolism when RNA splicing is interfered with. sgt13018 is such a mutant, with partial loss of function of GAMETOPHYTIC FACTOR 1 (GFA1); a gene likely involved in RNA biogenesis in Arabidopsis. The mutant is featured in the phenotype of diminished female gametophyte development at stage FG5 and is associated with the arrest of early embryo development in Arabidopsis. Bioinformatics data showed that homologs of gene GFA1 in yeast and human encode putative U5 snRNP-specific proteins required for pre-mRNA splicing. Furthermore, the result of yeast two-hybrid assay indicated that GFA1 physically interacted with AtBrr2 and AtPrp8, the putative U5 snRNP components, of Arabidopsis. This investigation suggests that GFA1 is involved in mRNA biogenesis through interaction with AtBrr2 and AtPrp8 and functions in megagametogenesis and embryogenesis in plant.
RNA生物合成对于基因的准确表达至关重要。显然，当RNA剪接受到干扰时，细胞无法继续正常代谢。sgt13018就是这样一个突变体，其配子体因子1（GFA1）功能部分丧失；GFA1是拟南芥中一个可能参与RNA生物合成的基因。该突变体的特征是在FG5阶段雌配子体发育减少的表型，并且与拟南芥早期胚胎发育的停滞有关。生物信息学数据表明，酵母和人类中GFA1基因的同源物编码前体mRNA剪接所需的假定U5 snRNP特异性蛋白。此外，酵母双杂交试验结果表明，GFA1与拟南芥假定的U5 snRNP组分AtBrr2和AtPrp8发生物理相互作用。这项研究表明，GFA1通过与AtBrr2和AtPrp8相互作用参与mRNA生物合成，并在植物的大配子发生和胚胎发生中发挥作用。
