Ishikawa Kiyoshi, Segawa Tatsuya, Hagiwara Yoshiaki, Maeda Masahiro, Abe Masaaki, Hino Okio
Immuno-bioligical Laboratories, Takasakishi, Gunma, Japan.
Pathol Int. 2009 Mar;59(3):161-6. doi: 10.1111/j.1440-1827.2009.02344.x.
Malignant mesothelioma is a highly aggressive tumor of the serosal cavity that arises from the mesothelial cells of the pleura, peritoneum, or pericardium. The immunohistochemical diagnosis of epithelioid mesothelioma from biopsy or surgically resected specimens has been actively pursued, using markers such as mesothelin. Several markers have indeed been helpful for confirming the diagnosis of mesothelioma and distinguishing between mesothelioma and adenocarcinoma. The authors have developed a novel mAb to human C-ERC/mesothelin, which performed well when used in western blotting, fluorescence-activated cell sorting, immunocytochemistry and immunohistochemistry, and which therefore will be useful in studying the molecular biology of mesothelin, in addition to improving the diagnosis and therapy of mesothelin-expressing cancers.
恶性间皮瘤是一种发生于浆膜腔的侵袭性很强的肿瘤,起源于胸膜、腹膜或心包膜的间皮细胞。利用间皮素等标志物,人们一直在积极探索通过活检或手术切除标本对上皮样间皮瘤进行免疫组化诊断。确实有几种标志物有助于确诊间皮瘤,并区分间皮瘤和腺癌。作者研发了一种针对人C-ERC/间皮素的新型单克隆抗体,该抗体在蛋白质印迹法、荧光激活细胞分选、免疫细胞化学和免疫组织化学中表现良好,因此除了改善对表达间皮素的癌症的诊断和治疗外,还将有助于研究间皮素的分子生物学。