Segawa Tatsuya, Hagiwara Yoshiaki, Ishikawa Kiyoshi, Aoki Naoko, Maeda Masahiro, Shiomi Kazu, Hino Okio
Immuno-Biological Laboratories Co. Ltd., 5-1 Aramachi, Takasaki, Gunma 370-0831, Japan.
Biochem Biophys Res Commun. 2008 May 9;369(3):915-8. doi: 10.1016/j.bbrc.2008.02.114. Epub 2008 Mar 5.
ERC/mesothelin is expressed on the normal mesothelium and some cancers such as mesothelioma or ovarian carcinoma. A splicing isoform of ERC/mesothelin (known as SMRP), which has an 82-bp insertion and codes for a C-terminus with a hydrophilic, presumably soluble, tail instead of a GPI-anchoring signal, has been reported as a useful marker for the diagnosis of mesothelioma. However, the existence of SMRP has not yet been demonstrated in the serum of mesothelioma patients. To elucidate the existence of SMRP, we have established a new enzyme-linked immunosorbent assay (ELISA) system for SMRP. The ELISA study revealed that N- and C-ERC/mesothelin were detected in sera from mesothelioma patients, but not SMRP, even in these samples. This result showed that the SMRP detected with MESOMARK kit should be lack of soluble C-terminus and indistinguishable from C-ERC/mesothelin. Further study might be necessary to demonstrate the relationship between SMRP and mesothelin.
ERC/间皮素在正常间皮及某些癌症（如间皮瘤或卵巢癌）中表达。据报道，ERC/间皮素的一种剪接异构体（称为SMRP）有一个82碱基对的插入片段，编码一个带有亲水性、可能可溶的尾巴的C末端，而非糖基磷脂酰肌醇锚定信号，它是诊断间皮瘤的一个有用标志物。然而，尚未在间皮瘤患者血清中证实SMRP的存在。为阐明SMRP的存在情况，我们建立了一种针对SMRP的新型酶联免疫吸附测定（ELISA）系统。ELISA研究显示，在间皮瘤患者血清中检测到了N-ERC/间皮素和C-ERC/间皮素，但未检测到SMRP，即便在这些样本中也是如此。这一结果表明，用MESOMARK试剂盒检测到的SMRP应该缺乏可溶的C末端，且与C-ERC/间皮素无法区分。可能需要进一步研究来证明SMRP与间皮素之间的关系。
