Ishikawa Akio, Kato Takako, Susa Takao, Sano Akiko, Kato Yukio
Division of Life Science, Graduate School of Agriculture, Meiji University.
J Reprod Dev. 2009 Jun;55(3):278-82. doi: 10.1262/jrd.20179. Epub 2009 Mar 4.
We cloned a porcine ortholog of homeodomain transcription factor Msx1 from the porcine pituitary cDNA library. The amino acid sequence of Msx1 shows high conservation among mammalian species. RT-PCR for porcine fetal and postnatal pituitaries showed that Msx1 is already expressed at early fetal day 40, decreases to a low level before birth and then remarkably decreases after birth. On the other hand, Msx1 expression was observed in all pituitary-derived cell line tested, with most in a gonadotrope lineage LbetaT4. Transfection assay demonstrated that Msx1 markedly repressed the basal Cga and Fshb gene expression, while Lhb expression was affected slightly. Taken together, Msx1 may play a role in repressing gene expression in the fetal and postnatal periods.
我们从猪垂体cDNA文库中克隆了同源结构域转录因子Msx1的猪直系同源基因。Msx1的氨基酸序列在哺乳动物物种间显示出高度保守性。对猪胎儿和出生后垂体进行的RT-PCR表明，Msx1在胎儿早期第40天就已表达，在出生前降至低水平，出生后则显著下降。另一方面，在所测试的所有垂体来源细胞系中均观察到Msx1表达，其中大多数表达于促性腺激素细胞系LbetaT4中。转染实验表明，Msx1显著抑制基础Cga和Fshb基因表达，而对Lhb表达的影响较小。综上所述，Msx1可能在胎儿期和出生后发挥抑制基因表达的作用。
