Marston Andrew, Hostettmann Kurt
Laboratory of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland.
Planta Med. 2009 Jun;75(7):672-82. doi: 10.1055/s-0029-1185379. Epub 2009 Mar 4.
Progress in natural product chemistry has always been strongly linked to innovations in analytical technology. The characterisation of metabolites in complex mixtures requires sophisticated techniques, which should provide good sensitivity and selectivity as well as structural information on the constituents of interest. This review outlines the most important chromatographic and spectral techniques which have been introduced in the field of natural products. Although there has been a very rapid evolution of methods over the last 50 years, the introduction of high-throughput screening programmes require even more efficient and sensitive methodologies which yield adequate on-line information for metabolite structure determination.
天然产物化学的进展一直与分析技术的创新紧密相连。复杂混合物中代谢物的表征需要精密的技术，这些技术应具备良好的灵敏度和选择性，以及有关目标成分的结构信息。本综述概述了天然产物领域中引入的最重要的色谱和光谱技术。尽管在过去50年里方法有了非常迅速的发展，但高通量筛选计划的引入需要更高效、更灵敏的方法，以便为代谢物结构测定提供足够的在线信息。
