Guzmán J M, Rubio M, Ortiz-Delgado J B, Klenke U, Kight K, Cross I, Sánchez-Ramos I, Riaza A, Rebordinos L, Sarasquete C, Zohar Y, Mañanós E L
Institute of Aquaculture of Torre Sal, Spanish Council for Scientific Research (CSIC), Torre Sal s/n, 12595-Cabanes, Castellón, Spain.
Comp Biochem Physiol A Mol Integr Physiol. 2009 Jul;153(3):266-77. doi: 10.1016/j.cbpa.2009.02.032. Epub 2009 Mar 2.
The Senegalese sole (Solea senegalensis) is a valuable flatfish for aquaculture, but it presents important reproductive problems in captivity. Spawning is achieved by wild-caught breeders but cultured broodstocks fail to spawn spontaneously and, when they do, eggs are unfertilized. To gain knowledge on the physiological basis underlying this reproductive dysfunction, this study aimed at analyzing comparative hormone levels between wild and cultured broodstocks at the spawning season. The Senegalese sole gonadotropin (GTH) subunits, FSHbeta, LHbeta and GPalpha, were cloned and qualitative (in situ hybridization) and quantitative (real-time PCR) assays developed to analyze pituitary GTH gene expression. In females, FSHbeta and GPalpha mRNA levels were higher in wild than in cultured broodstocks, whereas in males all three subunits were highest in cultured. By ELISA, three GnRH forms were detected in the pituitary, displaying a relative abundance of GnRH2>GnRH1>GnRH3. All GnRHs were slightly more abundant in wild than cultured females, whereas no differences were observed in males. Plasma levels of vitellogenin and sex steroids were also analyzed. Results showed endocrine differences between wild and cultured broodstocks at the spawning period, which could be related to the endocrine failure of the reproductive axis in cultured breeders.
塞内加尔鳎（Solea senegalensis）是水产养殖中一种有价值的比目鱼，但在圈养条件下存在重要的繁殖问题。野生捕获的亲鱼能够产卵，但养殖亲鱼不能自发产卵，即便产卵，卵也无法受精。为了解这种繁殖功能障碍的生理基础，本研究旨在分析产卵季节野生和养殖亲鱼之间的激素水平差异。克隆了塞内加尔鳎促性腺激素（GTH）亚基FSHβ、LHβ和GPα，并开发了定性（原位杂交）和定量（实时PCR）分析方法来分析垂体GTH基因表达。在雌性中，野生亲鱼的FSHβ和GPα mRNA水平高于养殖亲鱼，而在雄性中，所有三个亚基在养殖亲鱼中最高。通过酶联免疫吸附测定（ELISA），在垂体中检测到三种促性腺激素释放激素（GnRH）形式，其相对丰度为GnRH2>GnRH1>GnRH3。所有GnRH在野生雌性中的含量略高于养殖雌性，而在雄性中未观察到差异。还分析了血浆中卵黄蛋白原和性类固醇的水平。结果显示，产卵期野生和养殖亲鱼之间存在内分泌差异，这可能与养殖亲鱼生殖轴的内分泌功能衰竭有关。
