用于天然水体的一次性格式传感器中的多分析物成像。

Multianalyte imaging in one-shot format sensors for natural waters.

作者信息

Lapresta-Fernández A, Huertas Rafael, Melgosa Manuel, Capitán-Vallvey L F

机构信息

Solid Phase Spectrometry Research Group, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, E-18071 Granada, Spain.

出版信息

Anal Chim Acta. 2009 Mar 23;636(2):210-7. doi: 10.1016/j.aca.2009.01.044. Epub 2009 Jan 30.

DOI:10.1016/j.aca.2009.01.044

PMID:19264170

Abstract

A one-shot multisensor based on ionophore-chromoionophore chemistry for optical monitoring of potassium, magnesium and hardness in water is presented. The analytical procedure uses a black and white non-cooled CCD camera for image acquisition of the one-shot multisensor after reaction, followed by data treatment for quantitation using the grey value pixel average from a defined region of interest from each sensing area to build the analytical parameter 1-alpha. In optimised experimental conditions, the procedure shows a large linear range, up to 6 orders using the linearised model and good detection limits: 9.92 x 10(-5)mM, 1.86 x 10(-3)mM and 1.30 x 10(-2)mgL(-1) of CaCO(3) for potassium, magnesium and hardness, respectively. This analysis system exhibits good precision in terms of relative standard deviation (RSD%) from 2.3 to 3.8 for potassium, from 5.0 to 6.8 for magnesium and from 5.4 to 5.9 for hardness. The trueness of this multisensor procedure was demonstrated comparing it with results obtained by a DAD spectrophotometer used as a reference. Finally, it was satisfactorily applied to the analysis of these analytes in miscellaneous samples, such as water and beverage samples from different origins, validating the results against atomic absorption spectrometry (AAS) as the reference procedure.

摘要

本文介绍了一种基于离子载体-色离子载体化学的一次性多传感器，用于光学监测水中的钾、镁和硬度。分析过程使用黑白非制冷CCD相机在反应后采集一次性多传感器的图像，然后进行数据处理，通过从每个传感区域的定义感兴趣区域的灰度值像素平均值进行定量，以构建分析参数1-α。在优化的实验条件下，该方法显示出较大的线性范围，使用线性化模型可达6个数量级，且检测限良好：钾、镁和硬度的检测限分别为9.92×10⁻⁵mM、1.86×10⁻³mM和1.30×10⁻²mgL⁻¹（以碳酸钙计）。该分析系统在相对标准偏差（RSD%）方面表现出良好的精密度，钾的RSD%为2.3%至3.8%，镁的RSD%为5.0%至6.8%，硬度的RSD%为5.4%至5.9%。将该多传感器方法与用作参考的二极管阵列检测器（DAD）分光光度计获得的结果进行比较，证明了该方法的准确性。最后，该方法令人满意地应用于各种样品中这些分析物的分析，如不同来源的水和饮料样品，并以原子吸收光谱法（AAS）作为参考方法验证了结果。