Ohlsson K, Olsson I, Spitznagel K
Hoppe Seylers Z Physiol Chem. 1977 Mar;358(3):361-6. doi: 10.1515/bchm2.1977.358.1.361.
The subcellular localization of granulocyte collagenase, elastase and chymotrypsin-like cationic protein was determined using velocity centrifugation of cytoplasmic granules of human polymorphonuclear leukocytes. The proteases were assayed by immunochemical and enzymatic methods. Measurements of lactoferrin and myeloperoxidase distinguish exactly between constituents of specific and azurophil granules. Collagenase, elastase and chymotrypsin-like cationic proteins showed an almost identical sharp and unimodal distribution. They co-sedimented with myeloperoxidase demonstrating that these enzymes are localized exclusively in the azurophil granules.
利用人多形核白细胞胞质颗粒的速度离心法确定了粒细胞胶原酶、弹性蛋白酶和类胰凝乳蛋白酶阳离子蛋白的亚细胞定位。通过免疫化学和酶学方法对蛋白酶进行了测定。乳铁蛋白和髓过氧化物酶的测量能准确区分特异性颗粒和嗜天青颗粒的成分。胶原酶、弹性蛋白酶和类胰凝乳蛋白酶阳离子蛋白呈现出几乎相同的尖锐单峰分布。它们与髓过氧化物酶共同沉降，表明这些酶仅定位于嗜天青颗粒中。
