Deshpande Pallavi, Notara Maria, Bullett Nial, Daniels Julie T, Haddow David B, MacNeil Sheila
Department of Engineering Materials, Kroto Research Institute, University of Sheffield, Sheffield, UK.
Tissue Eng Part A. 2009 Oct;15(10):2889-902. doi: 10.1089/ten.tea.2008.0528.
Our aim was to develop an improved cell transfer system for delivering laboratory-cultured human limbal epithelial cells to the cornea, which would be low risk for the patient and convenient to use for the surgeon. We took a standard contact lens and developed a plasma polymer layer for coating this for attachment of cells to the lens and subsequent transfer of cells to the cornea. A range of plasma polymer surfaces were examined for initial cell attachment using three different combinations of human and rabbit epithelial and stromal cells, initially expanding cells both with and without bovine serum. The most promising surfaces, based on acrylic acid, were then coated onto contact lenses. Cell transfer from the lenses to the denuded surface of a 3D rabbit organ culture model was then used to make a second selection of substrates, which permitted reliable cell transfer. Primary rabbit and human corneal cells attached and proliferated well on acrylic acid-coated surfaces. Reliable transfer of primary epithelial cells from the coated contact lenses to a rabbit cornea was achieved by coating lenses with acrylic acid at 5 W/10 cm(3)/min and using cell densities of 1 x 10(5)/lens and above.
我们的目标是开发一种改进的细胞移植系统，用于将实验室培养的人角膜缘上皮细胞输送到角膜，该系统对患者风险低且对外科医生使用方便。我们采用了一种标准隐形眼镜，并开发了一种等离子体聚合物层来涂覆它，以便细胞附着在镜片上并随后将细胞转移到角膜。使用人和兔上皮细胞及基质细胞的三种不同组合，对一系列等离子体聚合物表面进行了初始细胞附着检查，最初在有和没有牛血清的情况下培养细胞。然后将基于丙烯酸的最有前景的表面涂覆在隐形眼镜上。然后利用从镜片到三维兔器官培养模型裸露表面的细胞转移来进行第二轮底物筛选，从而实现可靠的细胞转移。原代兔和人角膜细胞在丙烯酸涂层表面附着并良好增殖。通过以5W/10cm(3)/min的功率用丙烯酸涂覆镜片，并使用1×10(5)/镜片及以上的细胞密度，实现了原代上皮细胞从涂覆的隐形眼镜到兔角膜的可靠转移。
