1 Eye Hospital, Harbin Medical University , Nangang District, Harbin, Heilongjiang Province, China .
Tissue Eng Part A. 2014 Jun;20(11-12):1593-602. doi: 10.1089/ten.TEA.2013.0320. Epub 2014 Feb 27.
Surface treatment to a biomaterial surface has been shown to modify and help cell growth. Our aim was to determine the best surface-modified system for the treatment of limbal stem cell deficiency (LSCD), which would facilitate expansion of autologous limbal epithelial cells, while maintaining cultivated epithelial cells in a less differentiated state. Commercially available contact lenses (CLs) were variously surface modified by plasma polymerization with ratios of acrylic acid to octadiene tested at 100% acrylic acid, 50:50% acrylic acid:octadiene, and 100% octadiene to produce high-, mid-, and no-acid. X-ray photoelectron spectroscopy was used to analyze the chemical composition of the plasma polymer deposited layer. Limbal explants cultured on high acid-modified CLs outgrew more cells. Immunofluorescence and RT2-PCR array results indicated that a higher acrylic acid content can also help maintain progenitor cells during ex vivo expansion of epithelial cells. This study provides the first evidence for the ability of high acid-modified CLs to preserve the stemness and to be used as substrates for the culture of limbal cells in the treatment of LSCD.
生物材料表面的处理已被证明可以改变并有助于细胞生长。我们的目的是确定用于治疗角膜缘干细胞缺乏症（LSCD）的最佳表面改性系统，该系统将有助于扩增自体角膜缘上皮细胞，同时使培养的上皮细胞保持在较低分化状态。通过等离子体聚合对市售的隐形眼镜（CL）进行各种表面改性，并用 100%丙烯酸、50:50%丙烯酸：辛二烯和 100%辛二烯测试测试丙烯酸与辛二烯的比例，以产生高、中和低酸。使用 X 射线光电子能谱分析等离子体聚合物沉积层的化学成分。在高酸改性 CL 上培养的角膜缘外植体生长出更多的细胞。免疫荧光和 RT2-PCR 阵列结果表明，较高的丙烯酸含量也有助于在体外扩增上皮细胞过程中维持祖细胞。这项研究首次提供了高酸改性 CL 能够保存干细胞并可作为 LSCD 治疗中培养角膜缘细胞的基质的证据。
