Kosimov R B, Sukharev S I, Pospelova T V, Prudovskiĭ I A, Zelenin A V
Tsitologiia. 1991;33(2):48-55.
Heterokaryons between terminally differentiated polymorphonuclear leukocytes (PL) and culture cells of different proliferative potentials: mouse and rat embryo fibroblasts (EFM, EFR); immortal cells NIH 3T3 and E2; malignant cells NCC2, L929, He239 and SV 3T3,--were obtained by means of electrofusion. Radioautographic study of 3H-thymidine incorporation in the nuclei of heterokaryons showed that all the cells taken for fusion were able to induce reactivation of DNA synthesis in PL nuclei, however, with different rates: 7-37% for EFM and NIH 3T3 and 20-40% for malignant cells. The presence of oncogenes Elan in E2 cells and ras in NCC2 cells increased the rate of PL reactivation approximately twice as compared with the cells of original lines (EFR and NIH 3T3, correspondingly). In parallel to reactivation of DNA synthesis in PL nuclei inhibition of the synthesis in culture cell nuclei in the same heterokaryons was found. The rate of inhibition was about 70% for non-malignant and 23, 40 and 18% for NCC2, L and SV 3T3 cells, respectively. He239 cells, transformed by a temperature-dependent mutant of virus SV40 showed at permissive temperature the increased capacity of inducing reactivation of PL nuclei, though He239 cells susceptibility to inhibitory action of PL nuclei did not change with temperature. According to the behaviour in heterokaryons PL were found to be similar to chick erythrocytes, but differing from them by a pronounced inhibiting effect upon DNA synthesis in the nuclei of malignant cells.
通过电融合获得了终末分化的多形核白细胞（PL）与具有不同增殖潜能的培养细胞之间的异核体：小鼠和大鼠胚胎成纤维细胞（EFM、EFR）；永生细胞NIH 3T3和E2；恶性细胞NCC2、L929、He239和SV 3T3。对异核体细胞核中3H-胸腺嘧啶核苷掺入的放射自显影研究表明，所有用于融合的细胞都能够诱导PL细胞核中DNA合成的重新激活，然而，速率不同：EFM和NIH 3T3为7%-37%，恶性细胞为20%-40%。E2细胞中癌基因Elan和NCC2细胞中ras的存在使PL重新激活的速率比原始细胞系（分别为EFR和NIH 3T3）提高了约两倍。与PL细胞核中DNA合成的重新激活同时，在相同的异核体中发现培养细胞核中合成受到抑制。非恶性细胞的抑制率约为70%，NCC2、L和SV 3T3细胞的抑制率分别为23%、40%和18%。由病毒SV40的温度依赖性突变体转化的He239细胞在允许温度下显示出诱导PL细胞核重新激活的能力增强，尽管He239细胞对PL细胞核抑制作用的敏感性不随温度变化。根据在异核体中的行为，发现PL与鸡红细胞相似，但不同之处在于对恶性细胞核中DNA合成有明显的抑制作用。
