Mi-1.2转录本在抗性番茄中普遍积累。
Mi-1.2 Transcripts Accumulate Ubiquitously in Resistant Lycopersicon esculentum.
作者信息
de Ilarduya O M, Kaloshian I
出版信息
J Nematol. 2001 Jun;33(2-3):116-20.
The tomato Mi-1.2 gene confers resistance against both root-knot nematodes and the potato aphid. Plants are resistant to nematodes early in root development. However, plants as old as 4 weeks are susceptible to aphid infestation. We monitored Mi-1.2 expression at the transcriptional level in resistant (Mi/Mi) and susceptible (mi/mi) tomato cultivars by means of RT-PCR. Mi-1.2 transcripts accumulated in seeds, roots, stems, leaves, flowers, and green fruits of uninfected 10-week-old resistant plants but were not expressed in the same organs from similar-age susceptible plants. Mi-1.2 RNAs in roots and leaves can be detected very early in development, and levels of transcripts do not change after either root-knot nematode or aphid attack.
番茄Mi-1.2基因对根结线虫和马铃薯蚜虫均具有抗性。植株在根系发育早期对根结线虫具有抗性。然而，4周龄的植株易受蚜虫侵害。我们通过RT-PCR在抗性（Mi/Mi）和敏感（mi/mi）番茄品种中监测Mi-1.2基因在转录水平的表达。Mi-1.2转录本在未感染的10周龄抗性植株的种子、根、茎、叶、花和绿色果实中积累，但在同龄敏感植株的相同器官中不表达。根和叶中的Mi-1.2 RNA在发育早期就能检测到，并且在受到根结线虫或蚜虫攻击后，转录本水平不会发生变化。
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