Vos P, Simons G, Jesse T, Wijbrandi J, Heinen L, Hogers R, Frijters A, Groenendijk J, Diergaarde P, Reijans M, Fierens-Onstenk J, de Both M, Peleman J, Liharska T, Hontelez J, Zabeau M
Keygene N.V., Agro Business Park, Wageningen, The Netherlands.
Nat Biotechnol. 1998 Dec;16(13):1365-9. doi: 10.1038/4350.
Mi-1, a Lycopersicon peruvianum gene conferring resistance to the agricultural pests, root-knot nematodes, and introgressed into tomato, has been cloned using a selective restriction fragment amplification based strategy. Complementation analysis of a susceptible tomato line with a 100 kb cosmid array yielded a single cosmid clone capable of conferring resistance both to the root-knot nematode Meloidogyne incognita and to an unrelated pathogen, the potato aphid Macrosiphum euphorbiae. This resistance was stable. The Mi-1 gene encodes a protein sharing structural features with the nucleotide-binding site leucine-rich repeat-containing type of plant resistance genes.
Mi-1是秘鲁番茄中的一个基因，它能使植物抵抗农业害虫根结线虫，该基因已被导入番茄中。我们采用基于选择性限制性片段扩增的策略克隆了Mi-1基因。用一个包含100 kb黏粒的文库对一个感病番茄品系进行互补分析，得到了一个单一的黏粒克隆，该克隆既能使植物抵抗根结线虫南方根结线虫，又能抵抗一种非相关病原体——马铃薯蚜虫大戟长管蚜。这种抗性是稳定的。Mi-1基因编码的蛋白质与含有核苷酸结合位点和富亮氨酸重复序列类型的植物抗性基因具有相同的结构特征。
