通过重组工程构建基因靶向载体。
Construction of gene-targeting vectors by recombineering.
作者信息
Lee Song-Choon, Wang Wei, Liu Pentao
机构信息
Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
出版信息
Methods Mol Biol. 2009;530:15-27. doi: 10.1007/978-1-59745-471-1_2.
Abstract
Recombineering is a technology that utilizes the efficient homologous recombination functions encoded by gamma phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.
摘要
重组工程是一种利用γ噬菌体编码的高效同源重组功能在大肠杆菌中操纵DNA的技术。重组工程极大地促进了敲除载体的构建，因为它允许人们选择任何基因组区域进行操作。我们在此描述一种基于重组工程的高效方案，用于制备小鼠条件性敲除靶向载体。
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本文引用的文献
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Nucleic Acids Res. 2007;35(8):e64. doi: 10.1093/nar/gkm163. Epub 2007 Apr 10.
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