Thompson Nancy L, Wang Xiang, Navaratnarajah Punya
Department of Chemistry, University of North Carolina at Chapel Hill, 27599-3290, USA.
J Struct Biol. 2009 Oct;168(1):95-106. doi: 10.1016/j.jsb.2009.02.013. Epub 2009 Mar 6.
In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving supported membranes. Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined.
本文描述了全内反射荧光相关光谱法(TIR-FCS)的概念基础和实验设计。除了各种不直接涉及支撑膜的应用外,还讨论了TIR-FCS迄今为止在支撑膜方面的一些应用。与TIR-FCS相关但技术上不等同的方法也进行了总结。概述了TIR-FCS的未来发展方向。