在左旋甲状腺素钠中检测和半定量估算甲状腺素和二碘甲状腺原氨酸。
Detection and semiquantitative estimation of thyroxine and diiodothyronine in liothyronine sodium.
作者信息
Cieri U R, Illuminati J C
出版信息
J Assoc Off Anal Chem. 1977 May;60(3):628-34.
A method is presented for the detection and semiquantitative estimation of thyroxine and diiodothyronine in liothyronine sodium. Thyroxine is detected by thin layer chromatography, using silica gel H plates developed in butanol-acetone-ammonia (30 + 55 + 15) and sprayed with a 2,7-dichlorofluorescein solution, and estimated by comparison with standard spots. For quantitative results, a larger amount of sample is applied as a streak on a fluorescent silica gel H plate. After a 5 hr development, the band is scraped off and extracted in a small volume of 0.1N NaOH; the extract is centrifuged, transferred to a 2 cm microcell, and examined spectrophotometrically. The diiodothyronine content of liothyronine sodium is estimated by liquid chromatography on a Bondapak C18 column with 0.01N sodium perchloratebutanol-acetonitrile (1000 +62 + 188) as the eluting solvent.
本文介绍了一种检测和半定量估算碘塞罗宁钠中甲状腺素和二碘甲状腺原氨酸的方法。采用硅胶H板，以丁醇 - 丙酮 - 氨水（30 + 55 + 15）展开，并用2,7 - 二氯荧光素溶液喷雾，通过薄层色谱法检测甲状腺素，并与标准斑点比较进行估算。为获得定量结果，将较大量样品以条带形式点样于荧光硅胶H板上。展开5小时后，刮下条带，用少量0.1N NaOH萃取；萃取液离心后转移至2 cm微型比色皿中，进行分光光度法检测。碘塞罗宁钠中二碘甲状腺原氨酸的含量通过在Bondapak C18柱上进行液相色谱法估算，以0.01N高氯酸钠 - 丁醇 - 乙腈（1000 + 62 + 188）作为洗脱溶剂。
Zotero 插件