汉防己甲素对百草枯急性中毒致肺损伤治疗作用的实验研究

[Experimental study on therapeutic efficacy of tetrandrine on lung injury induced by acute of paraquat poisoning].

作者信息

Zhu Qiu-Hong, Huang Jin-Xiang, Meng Cong-Shen, Wang Ming-Liang, Chen Wei, Zhang Xing

机构信息

National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2008 Oct;26(10):583-7.

PMID:19272249

Abstract

OBJECTIVE

To investigate the antagonistic efficacy of tetrandrine (TET) on lung injury induced by acute paraquat poisoning.

METHODS

Male Wistar rats were randomly divided into three groups (control group, non-treatment group and treatment group). The tetrandrine of 30 mg/kg was given by gastric lavage six hours after 32 rats were intraperitoneally injected with paraquat 15 mg/kg (treatment group). Then the same dose of tetrandrine was given once a day. Normal saline of the same volume was given by gastric lavage in another 32 rats intraperitoneally injected with paraquat 15 mg/kg (non-treatment group). Seven rats were intraperitoneally injected by normal saline as the control group. Levels of maleic dialdehyde (MDA), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in plasma and the lung homogenate of three groups were determined at 3 d, 7 d, 14 d and 21 d after exposure to paraquat. Histological changes of the lungs were observed.

RESULTS

The levels of MDA at 3 d both in plasma [(3.65 +/- 0.44) nmol/ml] and the lung homogenate [(9.54 +/- 0.92) nmol/mg pro] of the non-treatment group significantly increased compared with the control group (P < 0.01), the activities of GSH-Px and SOD in plasma at 3 d were significantly less than the control group (P < 0.05 or P < 0.01), the activities of GSH-Px (3 d, 7 d) and SOD (7 d, 14 d) in the lung homogenate were significantly less than the control group (P < 0.05). There were no significant difference in the levels of MDA both in plasma and the lung homogenate between the treatment group and the non-treatment group (P > 0.05). The SOD activities of treatment group on the third day was significantly increased compared with the non-treatment group (P < 0.01 or P < 0.05). Although the activities of GSH-Px in plasma and the lung homogenate of the treatment group on the third day were increased, there was no significantly difference compared with the non-treatment group (P > 0.05). The integral score of pulmonary fibrosis in the treatment group were significantly lower than in the non-treatment group (P < 0.01).

CONCLUSION

TET has antagonistic effect against acute toxicity of paraquat through significant reduction of pulmonary fibrosis.

摘要

目的

探讨粉防己碱（TET）对急性百草枯中毒所致肺损伤的拮抗作用。

方法

将雄性Wistar大鼠随机分为三组（对照组、未治疗组和治疗组）。32只大鼠腹腔注射15mg/kg百草枯6小时后，给予30mg/kg粉防己碱灌胃（治疗组），之后每天给予相同剂量。另外32只腹腔注射15mg/kg百草枯的大鼠给予等体积生理盐水灌胃（未治疗组）。7只大鼠腹腔注射生理盐水作为对照组。在接触百草枯后3天、7天、14天和21天，测定三组大鼠血浆及肺匀浆中丙二醛（MDA）水平、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性，并观察肺组织学变化。

结果

未治疗组3天时血浆中MDA水平[（3.65±0.44）nmol/ml]及肺匀浆中MDA水平[（9.54±0.92）nmol/mg蛋白]与对照组相比显著升高（P<0.01），3天时血浆中GSH-Px和SOD活性显著低于对照组（P<0.05或P<0.01），肺匀浆中GSH-Px（3天、7天）和SOD（7天、14天）活性显著低于对照组（P<0.05）。治疗组与未治疗组血浆及肺匀浆中MDA水平无显著差异（P>0.05）。治疗组第3天时SOD活性与未治疗组相比显著升高（P<0.01或P<0.05）。虽然治疗组第3天时血浆及肺匀浆中GSH-Px活性升高，但与未治疗组相比无显著差异（P>0.05）。治疗组肺纤维化积分显著低于未治疗组（P<0.01）。