[异丙酚对百草枯诱导的大鼠肺损伤的保护作用]

[Propofol protecting rats from paraquat induced lung injury].

作者信息

Shi Han-wen, Hu Jun-li, Tian Ying-ping, Huang Hong-mei, Li Xing-hai, Su Jian-ling

机构信息

Department of Emergency, Second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Jun;24(6):345-7.

PMID:16824339

Abstract

OBJECTIVE

To investigate the protective efficacy of propofol against paraquat induced lung injury.

METHODS

One hundred and twenty-eight male Wistar rats were randomizedly divided into three groups: the control group (n = 8), the intoxication group (n = 60) and the propofol group (n = 60). One hundred and twenty rats were once administered with 5 mg/kg paraquat (PQ) by the intragastrical injection to establish the model of PQ induced lung injury. The propofol of 10 mg/kg was administered intraperitoneally in the propofol group (60 rats) twice a day for four consecutive days one hour after the rats were intoxicated while the normal saline of the same amount as propofol in the propofol group was administered in the intoxication group (60 rats) one hour after the rats were intoxicated. The intragastrical injection of 1 mg/kg normal saline was administered once in the control group (8 rats). On the first, the third, the seventh, the 14th and the 28th day after treating, the level of malondialdehyde (MDA) in plasma, bronchoalveolar lavage fluid (BALF) and lung homogenate, and the content of hydroxyproline (HPY) in lung homogenate, the cell count of BALF were detected. Meanwhile, pathological changes of lung were examined under optical microscope.

RESULTS

The level of MDA in plasma on the first, the third and the seventh day and in BALF and lung homogenate on the first and the third day in the propofol group [in plasma: (4.31 +/- 0.94), (4.04 +/- 0.87) and (3.24 +/- 1.14) nmol/ml; in BALF: (3.47 +/- 1.09) and (2.79 +/- 1.11) nmol/ml; in lung homogenate: (7.54 +/- 0.63) and (8.41 +/- 1.23) nmol/ml] were significantly lower than those in the intoxication group [in plasma: (10.15 +/- 3.15), (6.97 +/- 1.6 5) and (5.44 +/- 0.66) nmol/ml; in BALF: (5.58 +/- 1.19) and (4.86 +/- 1.89) nmol/ml; in lung homogenate: (10.20 +/- 2.43) and (10.71 +/- 171) nmol/ml, P < 0.05 or P < 0.01]. The total cell count of BALF on the first, the third and the seventh day after intoxication in the propofol group was significantly less than that in the intoxication group respectively (P < 0.05). The histological changes such as alveolar edema, hemorrhage and inflammatory cell infiltration in the propofol group were less than those in the PQ group.

CONCLUSION

Propofol could reduce the level of MDA and relieve paraquat induced lung injury.

摘要

目的

探讨丙泊酚对百草枯诱导的肺损伤的保护作用。

方法

将128只雄性Wistar大鼠随机分为三组：对照组（n = 8）、中毒组（n = 60）和丙泊酚组（n = 60）。120只大鼠经胃内注射5 mg/kg百草枯（PQ）建立PQ诱导的肺损伤模型。丙泊酚组（60只大鼠）在中毒后1小时腹腔注射10 mg/kg丙泊酚，连续4天，每天2次；中毒组（60只大鼠）在中毒后1小时注射与丙泊酚组等量的生理盐水。对照组（8只大鼠）经胃内注射1 mg/kg生理盐水1次。治疗后第1、3、7、14和28天，检测血浆、支气管肺泡灌洗液（BALF）和肺匀浆中丙二醛（MDA）水平、肺匀浆中羟脯氨酸（HPY）含量、BALF细胞计数。同时，在光学显微镜下观察肺组织病理变化。

结果

丙泊酚组中毒后第1、3、7天血浆中MDA水平及中毒后第1、3天BALF和肺匀浆中MDA水平[血浆：（4.31±0.94）、（4.04±0.87）和（3.24±1.14）nmol/ml；BALF：（3.47±1.09）和（2.79±1.11）nmol/ml；肺匀浆：（7.54±0.63）和（8.41±1.23）nmol/ml]显著低于中毒组[血浆：（10.15±3.15）、（6.97±1.65）和（5.44±0.66）nmol/ml；BALF：（5.58±1.19）和（4.86±1.89）nmol/ml；肺匀浆：（10.20±2.43）和（10.71±1.71）nmol/ml，P<0.05或P<0.01]。丙泊酚组中毒后第1、3、7天BALF总细胞计数分别显著低于中毒组（P<0.05）。丙泊酚组肺泡水肿、出血和炎症细胞浸润等组织学改变较PQ组轻。