端粒酶和可溶性Fas在与血吸虫病相关的各种膀胱病变发病机制中的可能作用。

Possible role of telomerase and sFas in pathogenesis of various bladder lesions associated with schistosomiasis.

作者信息

Shaker Olfat G, Hammam Olfat, Salehd Abeya, el-Leithy Tarek, Wishahi Mohamed

机构信息

Department of Cairo University, Cairo, Egypt.

出版信息

Clin Biochem. 2009 Jun;42(9):864-72. doi: 10.1016/j.clinbiochem.2008.12.025. Epub 2009 Jan 14.

DOI:10.1016/j.clinbiochem.2008.12.025

PMID:19272297

Abstract

OBJECTIVES

To find the role of telomerase and sFas in the pathogenesis of various bladder lesions associated with schistosomiasis and to correlate the results with clinicopathological parameters.

DESIGN AND METHODS

One hundred bladder samples were taken, including 65 cases with bladder cancer, 25 cases with chronic cystitis and 10 samples from normal bladder tissue as control. Telomerase activity was measured using TRAP and hTERT techniques. Also, sFas was detected by ELISA technique in serum of all patients.

RESULTS

TRAP activity was detected in 78.5%; there was a significant increase in the number of positive cases in schistosomal urothelial carcinoma (TCC) and squamous cell carcinoma (SCC) compared to control and non-schistosomal urothelial carcinoma at (p<0.01 for each). TRAP activity was positive in 100% of high grade urothelial carcinoma compared to low grade and 92% positive in invasive tumors compared to non invasive tumors. hTERT protein was detected in 75.4% of bladder cancer cases; there was a significant increase in the number of positive cases in schistosomal urothelial carcinoma and SCC compared to control and non schistosomal urothelial carcinoma (p<0.01 for each). hTERT was positive in 100% of high grade and invasive TCC. sFas was detected in 64.6% in bladder cancer cases; there was a significant increase in the number of positive cases in SCC compared to control and non-schistosomal urothelial carcinoma.

CONCLUSION

There is an increase in telomerase activity and over-expression of hTERT proteins in schistosomal associated bladder cancer (SABC) in comparison to non schistosomal associated bladder cancer (non SABC). Also, there is an increase in sFas level in SCC compared to other the groups. Both, telomerase activity by TRAP and hTERT and sFas may be of significance in the development of SABC. They may also be useful markers to identify bladder carcinoma through telomerase inhibition.

摘要

目的

探讨端粒酶和可溶性 Fas（sFas）在血吸虫病相关各种膀胱病变发病机制中的作用，并将结果与临床病理参数进行关联分析。

设计与方法

采集 100 份膀胱样本，其中包括 65 例膀胱癌患者样本、25 例慢性膀胱炎患者样本以及 10 份正常膀胱组织样本作为对照。采用端粒重复序列扩增法（TRAP）和人端粒酶逆转录酶（hTERT）技术检测端粒酶活性。此外，通过酶联免疫吸附测定（ELISA）技术检测所有患者血清中的 sFas。

结果

78.5%的样本检测到端粒酶重复序列扩增法（TRAP）活性；与对照组和非血吸虫性尿路上皮癌相比，血吸虫性尿路上皮癌（移行细胞癌，TCC）和鳞状细胞癌（SCC）的阳性病例数显著增加（每组 p<0.01）。与低级别尿路上皮癌相比，高级别尿路上皮癌的端粒酶重复序列扩增法（TRAP）活性阳性率为 100%；与非浸润性肿瘤相比，浸润性肿瘤的阳性率为 92%。75.4%的膀胱癌病例检测到 hTERT 蛋白；与对照组和非血吸虫性尿路上皮癌相比，血吸虫性尿路上皮癌和鳞状细胞癌的阳性病例数显著增加（每组 p<0.01）。高级别和浸润性移行细胞癌的 hTERT 阳性率为 100%。64.6%的膀胱癌病例检测到 sFas；与对照组和非血吸虫性尿路上皮癌相比，鳞状细胞癌的阳性病例数显著增加。

结论

与非血吸虫性相关膀胱癌（非 SABC）相比，血吸虫性相关膀胱癌（SABC）中端粒酶活性增加，hTERT 蛋白过度表达。此外，与其他组相比，鳞状细胞癌中的 sFas 水平升高。端粒酶重复序列扩增法（TRAP）检测的端粒酶活性、hTERT 和 sFas 在血吸虫性相关膀胱癌（SABC）的发生发展中可能具有重要意义。它们也可能是通过抑制端粒酶来识别膀胱癌的有用标志物。