碱烧伤角膜伤口愈合过程中肌成纤维细胞对基质金属蛋白酶（MMP）-12的表达
Expression of matrix metalloproteinases (MMP)-12 by myofibroblasts during alkali-burned corneal wound healing.
作者信息
Iwanami Haruhi, Ishizaki Masamichi, Fukuda Yuh, Takahashi Hiroshi
机构信息
Department of Ophthalmology, Nippon Medical School, Bunkyo-ku, Tokyo, Japan.
出版信息
Curr Eye Res. 2009 Mar;34(3):207-14. doi: 10.1080/02713680802687809.
PURPOSE
The purpose of this study was to determine the expression of MMP-12 by myofibroblasts during the healing of alkali-burned rabbit corneas (ARC), thus implicating its role in ECM remodeling.
METHODS
Rabbit corneas during alkali burn were examined for MMP-12 mRNA expression by RT-PCR. Immunohistochemistry was used to determine the presence of alpha-SMA, MMP-12 protein, and macrophages. In situ hybridization was performed to identify MMP-12 mRNA expressing cells.
RESULTS
RT-PCR showed that MMP-12 mRNA was expressed in the alkali-burned corneas from one week after the injury. Immunohistochemistry showed myofibroblasts positive for MMP-12 expression. In situ hybridization revealed that MMP-12 mRNA was expressed by myofibroblasts.
CONCLUSION
Our results indicate that, in alkali-burned corneas, myofibroblasts express both MMP-12 mRNA and protein. We suggest that MMP-12 may disintegrate some components of the ECM released after severe alkali burn, which may be involved in the ECM remodeling.
目的
本研究旨在确定碱烧伤兔角膜（ARC）愈合过程中肌成纤维细胞MMP - 12的表达情况，进而探讨其在细胞外基质（ECM）重塑中的作用。
方法
采用逆转录 - 聚合酶链反应（RT - PCR）检测碱烧伤兔角膜中MMP - 12 mRNA的表达。免疫组织化学法用于确定α - 平滑肌肌动蛋白（α - SMA）、MMP - 12蛋白及巨噬细胞的存在情况。原位杂交用于鉴定表达MMP - 12 mRNA的细胞。
结果
RT - PCR显示，损伤后1周起碱烧伤角膜中即有MMP - 12 mRNA表达。免疫组织化学显示肌成纤维细胞MMP - 12表达呈阳性。原位杂交显示MMP - 12 mRNA由肌成纤维细胞表达。
结论
我们的结果表明，在碱烧伤角膜中，肌成纤维细胞同时表达MMP - 12 mRNA和蛋白。我们认为MMP - 12可能分解严重碱烧伤后释放的ECM的某些成分，这可能参与了ECM重塑。
Zotero 插件