Prinsloo Earl, Setati Mokgadi M, Longshaw Victoria M, Blatch Gregory L
Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown, South Africa.
Bioessays. 2009 Apr;31(4):370-7. doi: 10.1002/bies.200800158.
Self-renewal and differentiation of stem cells are tightly regulated processes subject to intrinsic and extrinsic signals. Molecular chaperones and co-chaperones, especially heat shock proteins (Hsp), are ubiquitous molecules involved in the modulation of protein conformational and complexation states. The function of Hsp, which are typically associated with stress response and tolerance, is well characterized in differentiated cells, while their role in stem cells remains unclear. It appears that embryonic stem cells exhibit increased stress tolerance and concomitant high levels of chaperone expression. This review critically evaluates stem cell research from a molecular chaperone perspective. Furthermore, we propose a model of chaperone-modulated self-renewal in mouse embryonic stem cells.
干细胞的自我更新和分化是受到内在和外在信号严格调控的过程。分子伴侣和辅助分子伴侣，尤其是热休克蛋白（Hsp），是参与调节蛋白质构象和复合状态的普遍存在的分子。Hsp的功能通常与应激反应和耐受性相关，在分化细胞中已得到充分表征，但其在干细胞中的作用仍不清楚。胚胎干细胞似乎表现出增强的应激耐受性以及伴随的高水平伴侣蛋白表达。本综述从分子伴侣的角度批判性地评估了干细胞研究。此外，我们提出了一个分子伴侣调节小鼠胚胎干细胞自我更新的模型。
